Revealing physiological and genetic properties of a dominant maize dwarf Dwarf11 (D11) by integrative analysis

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• 作者：Yijun Wang ; Wenjie Lu ; Yao Chen ; Dexiang Deng ; Haidong Ding…
• 关键词：D11 ; Dominant dwarf ; Gibberellin ; Genetic modifier ; Maize (Zea mays L.)
• 刊名：Molecular Breeding
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：36
• 期：3
• 全文大小：1,473 KB
• 参考文献：Cassani E, Bertolini E, Badone FC, Landoni M, Gavina D, Sirizzotti A, Pilu R (2009) Characterization of the first dominant dwarf maize mutant carrying a single amino acid insertion in the VHYNP domain of the dwarf8 gene. Mol Breed 24:375–385CrossRef
  Chen Y, Hou M, Liu L, Wu S, Shen Y, Ishiyama K, Kobayashi M, McCarty DR, Tan BC (2014) The maize DWARF1 encodes a gibberellin 3-oxidase and is dual localized to the nucleus and cytosol. Plant Physiol 166:2028–2039CrossRef PubMed PubMedCentral
  Fujioka S, Yamane H, Spray CR, Gaskin P, Macmillan J, Phinney BO, Takahashi N (1988a) Qualitative and quantitative analyses of gibberellins in vegetative shoots of normal, dwarf-1, dwarf-2, dwarf-3, and dwarf-5 seedlings of Zea mays L. Plant Physiol 88:1367–1372CrossRef PubMed PubMedCentral
  Fujioka S, Yamane H, Spray CR, Katsumi M, Phinney BO, Gaskin P, Macmillan J, Takahashi N (1988b) The dominant non-gibberellin-responding dwarf mutant (D8) of maize accumulates native gibberellins. Proc Natl Acad Sci USA 85:9031–9035CrossRef PubMed PubMedCentral
  Hedden P (2003) The genes of the Green Revolution. Trends Genet 19:5–9CrossRef PubMed
  Khush GS (2001) Green revolution: the way forward. Nat Rev Genet 2:815–822CrossRef PubMed
  Lawit SJ, Wych HM, Xu D, Kundu S, Tomes DT (2010) Maize DELLA proteins dwarf plant8 and dwarf plant9 as modulators of plant development. Plant Cell Physiol 51:1854–1868CrossRef PubMed
  Li QC, Li YX, Yang ZZ, Liu C, Liu ZZ, Li CH, Peng B, Zhang Y, Wang D, Tan WW, Sun BC, Shi YS, Song YC, Zhang ZM, Pan GT, Wang TY, Li Y (2013) QTL mapping for plant height and ear height by using multiple related RIL populations in maize. Acta Agron Sin 39:1521–1529CrossRef
  Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25:402–408CrossRef PubMed
  Lv H, Zheng J, Wang T, Fu J, Huai J, Min H, Zhang X, Tian B, Shi Y, Wang G (2014) The maize d2003, a novel allele of VP8, is required for maize internode elongation. Plant Mol Biol 84:243–257CrossRef PubMed
  Makarevitch I, Thompson A, Muehlbauer GJ, Springer NM (2012) Brd1 gene in maize encodes a brassinosteroid C-6 oxidase. PLoS ONE 7:e30798CrossRef PubMed PubMedCentral
  Middleton AM, Úbeda-Tomás S, Griffiths J, Holman T, Hedden P, Thomas SG, Phillips AL, Holdsworth MJ, Bennett MJ, King JR, Owen MR (2012) Mathematical modeling elucidates the role of transcriptional feedback in gibberellin signaling. Proc Natl Acad Sci USA 109:7571–7576CrossRef PubMed PubMedCentral
  Multani DS, Briggs SP, Chamberlin MA, Blakeslee JJ, Murphy AS, Johal GS (2003) Loss of an MDR transporter in compact stalks of maize br2 and sorghum dw3 mutants. Science 302:81–84CrossRef PubMed
  Peiffer JA, Romay MC, Gore MA, Flint-Garcia SA, Zhang Z, Millard MJ, Gardner CA, McMullen MD, Holland JB, Bradbury PJ, Buckler ES (2014) The genetic architecture of maize height. Genetics 196:1337–1356CrossRef PubMed PubMedCentral
  Peng J, Richards DE, Hartley NM, Murphy GP, Devos KM, Flintham JE, Beales J, Fish LJ, Worland AJ, Pelica F, Sudhakar D, Christou P, Snape JW, Gale MD, Harberd NP (1999) ‘Green revolution’ genes encode mutant gibberellin response modulators. Nature 400:256–261CrossRef PubMed
  Pilu R, Cassani E, Villa D, Curiale S, Panzeri D, Cerino Badone F, Landoni M (2007) Isolation and characterization of a new mutant allele of brachytic 2 maize gene. Mol Breed 20:83–91CrossRef
  Salas Fernandez MG, Becraft PW, Yin Y, Lübberstedt T (2009) From dwarves to giants? Plant height manipulation for biomass yield. Trends Plant Sci 14:454–461CrossRef PubMed
  Song J, Guo B, Song F, Peng H, Yao Y, Zhang Y, Sun Q, Ni Z (2011) Genome-wide identification of gibberellins metabolic enzyme genes and expression profiling analysis during seed germination in maize. Gene 482:34–42CrossRef PubMed
  Teng F, Zhai L, Liu R, Bai W, Wang L, Huo D, Tao Y, Zheng Y, Zhang Z (2013) ZmGA3ox2, a candidate gene for a major QTL, qPH3.1, for plant height in maize. Plant J 73:405–416CrossRef PubMed
  Wang Y, Deng D (2014) Molecular basis and evolutionary pattern of GA-GID1-DELLA regulatory module. Mol Genet Genomics 289:1–9CrossRef PubMed
  Wang Y, Deng D, Ding H, Xu X, Zhang R, Wang S, Bian Y, Yin Z, Chen Y (2013) Gibberellin biosynthetic deficiency is responsible for maize dominant Dwarf11 (D11) mutant phenotype: physiological and transcriptomic evidence. PLoS ONE 8:e66466CrossRef PubMed PubMedCentral
  Weng J, Xie C, Hao Z, Wang J, Liu C, Li M, Zhang D, Bai L, Zhang S, Li X (2011) Genome-wide association study identifies candidate genes that affect plant height in Chinese elite maize (Zea mays L.) inbred lines. PLoS ONE 6:e29229CrossRef PubMed PubMedCentral
  Winkler RG, Helentjaris T (1995) The maize Dwarf3 gene encodes a cytochrome P450-mediated early step in gibberellin biosynthesis. Plant Cell 7:1307–1317CrossRef PubMed PubMedCentral
  Xing A, Gao Y, Ye L, Zhang W, Cai L, Ching A, Llaca V, Johnson B, Liu L, Yang X, Kang D, Yan J, Li J (2015) A rare SNP mutation in Brachytic2 moderately reduces plant height and increases yield potential in maize. J Exp Bot 66:3791–3802CrossRef PubMed PubMedCentral
  Xu YJ, Gu DJ, Zhang BB, Zhang H, Wang ZQ, Yang JC (2013) Hormone contents in kernels at different positions on an ear and their relationship with endosperm development and kernel filling in maize. Acta Agron Sin 39:1452–1461CrossRef
  Yamaguchi S (2008) Gibberellin metabolism and its regulation. Annu Rev Plant Biol 59:225–251CrossRef PubMed
  Zhang S, Liu F, Liu B, Wang L, Dong S (2007) Discovery of a new dominant dwarf gene in maize and its preliminary study. J Maize Sci 15:15–18
  Zheng DB, Yang XH, Li JS, Yan JB, Zhang SL, He ZH, Huang YQ (2013) QTL identification for plant height and ear height based on SNP mapping in maize (Zea mays L.). Acta Agron Sin 39:549–556CrossRef
  
• 作者单位：Yijun Wang (1) (2)
  Wenjie Lu (1)
  Yao Chen (1)
  Dexiang Deng (1)
  Haidong Ding (3)
  Yunlong Bian (1)
  Zhitong Yin (1)
  Ya Zhu (1)
  Jia Zhao (1)
  
  1. Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou, 225009, China
  2. Shanghai Key Laboratory of Bio-Energy Crops, Shanghai, 200444, China
  3. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, 225009, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  
• 出版者：Springer Netherlands
• ISSN：1572-9788

文摘

Plant height is an important agronomic trait involved in lodging resistance and harvest index. The identification and characterization of mutants that are defective in plant height have implications for trait improvement in breeding programs. Two dominant maize dwarf mutants D8 and D9 have been well-characterized. Here, we report the characterization of a dominant maize dwarf mutant Dwarf11 (D11). Dwarf stature of D11 was mainly attributed to the inhibition of longitudinal cell elongation. The levels of bioactive GA₃ were significantly lower in D11. Contrarily, D8 mutant accumulates markedly higher levels of GA₃. The expression of GA biosynthetic and catabolic genes was dramatically decreased in D11. Expression variations of d8 and d9 genes were not observed in D11 mutant. Moreover, genetic suppressors of D11 were identified in inbred line Chang 7-2. Integrated omics data indicated that D11 is a novel dominant maize dwarf. The ultimate D11 gene cloning and its regulatory network elucidation may strengthen our understanding of the genetic basis of plant architecture and provide cues for breeding of crops with plant height ideotypes.