Antiepileptogenic effects of borneol in pentylenetetrazole-induced kindling in mice

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• 作者：Rufi Tambe ; Pankaj Jain ; Sachin Patil…
• 关键词：Kindling ; Borneol ; Pentylenetetrazole ; Oxidative Stress ; Glial fibrillary acidic protein
• 刊名：Naunyn-Schmiedeberg's Archives of Pharmacology
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：389
• 期：5
• 页码：467-475
• 全文大小：750 KB
• 参考文献：Aebi H (1974) Catalase. In: Bergmeyer HU (ed) Methods in enzymatic analysis, vol 3. Academic Press Inc, New York, pp. 673–686CrossRef
  Almeida JR, Souza GR, Silva JC, Saraiva SR, Oliveira Júnior RG, Quintans JS, Barreto RS, Bonjardim LR, Cavalcanti SC, Quintans Júnior LJ (2013) Borneol, a bicyclic monoterpene alcohol, reduces nociceptive behavior and inflammatory response in mice. Scientific World Journal. Hindawi Publishing Corporation 1-5
  Cavazos JE, Sutula TP (1990) Progressive neuronal loss induced by kindling: a possible mechanism for mossy fiber synaptic reorganization and hippocampal sclerosis. Brain Res 527(1):1–6CrossRef PubMed
  Costa LG (1994) Cell signaling and neurotoxic events. In: Chang LW, editor. Principles of neurotoxicology. New York: Marcel Dekker, 475–493.
  Dringen R, Gutterer JM, Hirrlinger J (2000) Glutathione metabolism in brain metabolic interaction between astrocytes and neurons in the defense against reactive oxygen species. Eur J Biochem 267(16):4912–4916CrossRef PubMed
  Franke H, Kittner H (2001) Morphological alterations of neurons and astrocytes and changes in emotional behavior in pentylenetetrazol-kindled rats. Pharmacol Biochem Behav. 70(2–3):291–303CrossRef PubMed
  Frantseva MV, Perez Velazquez JL, Tsoraklidis G, Mendonca AJ, Adamchik Y, Mills LR, Carlen PL, Burnham MW (2000) Oxidative stress is involved in seizure-induced neurodegeneration in the kindling model of epilepsy. Neuroscience 97(3):431–435CrossRef PubMed
  Granger RE, Campbell EL, Johnston GA (2005) (+)- and (−)-Borneol: efficacious positive modulators of GABA action at human recombinant α1β2γ2L GABAA receptors. Biochem Pharmacol 69(7):1101–1111CrossRef PubMed
  Gupta YK, Veerendra Kumar MH, Srivastava AK (2003) Effect of Centella asiatica on pentylenetetrazole-induced kindling, cognition and oxidative stress in rats. Pharmacol Biochem Behav. 74(3):579–585CrossRef PubMed
  Horváthová E, Slamenová D, Marsálková L, Sramková M, Wsólová L (2009) Effects of borneol on the level of DNA damage induced in primary rat hepatocytes and testicular cells by hydrogen peroxide. Food Chem Toxicol 47(6):1318–1323CrossRef PubMed
  Ilhan A, Ahmet G, Ferah A, Suat K, Mustafa I (2005) Antiepileptogenic and antioxidant effects of Nigella sativa oil against pentylenetetrazole induced kindling in mice. Neuropharmacology 49(4):456–464CrossRef PubMed
  Ishige K, Schubert D, Sagara Y (2001) Flavonoids protect neuronal cells from oxidative stress by three distinct mechanisms. Free Radic Biol Med 30(4):433–446CrossRef PubMed
  Jain S, Bharal N, Khurana S, Mediratta PK, Sharma KK (2011) Anticonvulsant and antioxidant actions of trimetazidine in pentylenetetrazole-induced kindling model in mice. Naunyn-Schmied Arch Pharmacol 383:385–392CrossRef
  Khurgel M, Ivy GO (1996) Astrocytes in kindling: relevance to epileptogenesis. Epilepsy Res 26(1):163–175CrossRef PubMed
  Li X (2012) Improved pyrogallol autoxidation method: a reliable and cheap superoxide scavenging assay suitable for all antioxidants. J Agric Food Chem 60(25):6418–6424CrossRef PubMed
  Liu R, Zhang L, Lan X, Li L, Zhang TT, Sun JH, Du GH (2011) Protection by borneol on cortical neurons against oxygen-glucose deprivation/reperfusion: involvement of anti-oxidation and anti-inflammation through nuclear transcription factor κappaB signaling pathway. Neuroscience 176:408–419CrossRef PubMed
  Löscher W, Schmidt D (2011) Modern antiepileptic drug development has failed to deliver: ways out of the current dilemma. Epilepsia 52(4):657–678CrossRef PubMed
  Lucindo J, Guimarães A, Araújo B, Oliveira G, Santana M, Moreira F, Santos M, Cavalcanti S, Lucca W, Botelho M, Ribeiro L, Nóbrega F, Almeida R (2010) Carvacrol, (−)-borneol and citral reduce convulsant activity in rodents. Afr J Biotechnol 9(39):6566–6572
  Matsuda S, Umeda M, Uchida H, Kato H, Araki T (2009) Alterations of oxidative stress markers and apoptosis markers in the striatum after transient focal cerebral ischemia in rats. J Neural Transm 116(4):395–404CrossRef PubMed
  Nandi A, Chatterjee B (1988) Assay of superoxide dismutase activity in animal tissues. J Biosci 13(3):305–315CrossRef
  Okhawa H, Nobuko O, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95(2):351–358CrossRef
  Pohle W, Becker A, Grecksch G, Juhre A, Willenberg A (1997) Piracetam prevents pentylenetetrazol kindling-induced neuronal loss and learning deficits. Seizure 6(6):467–474CrossRef PubMed
  Rauca C, Zerbe R, Jantze H (1999) Formation of free hydroxyl radicals after pentylenetetrazole-induced seizure and kindling. Brain Res 847(2):347–351CrossRef PubMed
  Reilly CA, Aust SD (1999) Measurement of lipid peroxidation. Curr Protoc Toxicol 2(4):1–13
  Saha L, Bhandari S, Bhatia A, Banerjee D, Chakrabarti A (2014) Anti-kindling effect of bezafibrate, a peroxisome proliferator-activated receptors alpha agonist, in pentylenetetrazole induced kindling seizure model. J Epilepsy Res 4(2):45–54CrossRef PubMed PubMedCentral
  Sakuma M, Hayakawa N, Kato H, Araki T (2008) Time dependent changes of striatal interneurons after focal cerebral ischemia. J Neural Transm 115(3):413–422CrossRef PubMed
  Schmidt D (2002) The clinical impact of new antiepileptic drugs after a decade of use in epilepsy. Epilepsy Res 50(1–2):21–32CrossRef PubMed
  Sedlak J, Lindsay RH (1968) Estimation of total and non-protein sulfhydryl groups in tissues with Ellman’s reagent. Anal Biochem 25(1):192–205CrossRef PubMed
  Sherafat MA, Ronaghi A, Molaei L, Nejadhoseynian M, Ghasemi R, Hosseini A, Naderi N, Motamedi F (2013) Kindling-induced learning deficiency and possible cellular and molecular involved mechanisms. Neurol Sci 34(6):883–890CrossRef PubMed
  Silveira e Sá R, LN A, de Sousa D P (2013) A review on anti-inflammatory activity of monoterpenes. Molecules 18(1):1227–1254CrossRef
  Sinha AK (1972) Calorimetric assay of catalase. Anal Biochem 47(2):389–394CrossRef PubMed
  Sleven H, Gibbs J, Heales S, Thom M, Cock HR (2006) Depletion of reduced glutathione precedes inactivation of mitochondrial enzymes following limbic status epilepticus in the rat hippocampus. Neurochem Int 48(2):75–82CrossRef PubMed
  Smith IK, Vierheller TL, Thorne CA (1988) Assay of glutathione reductase in crude tissue homogenates using 5,5′-dithiobis(2-nitrobenzoic acid). Anal Biochem 175(2):408–413CrossRef PubMed
  Weydert CJ, Cullen JJ (2010) Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue. Nat Protoc 5(1):51–66CrossRef PubMed PubMedCentral
  
• 作者单位：Rufi Tambe (1)
  Pankaj Jain (1)
  Sachin Patil (1)
  Priya Ghumatkar (1)
  Sadhana Sathaye (1)
  
  1. Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai-19, Maharashtra, India
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Pharmacology and Toxicology
  Neurosciences
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1912

文摘

Borneol, a bicyclic monoterpene, can easily cross the blood brain barrier and was found to possess gamma amino butyric acid (GABA) modulatory effect. The present study was aimed at investigating the antiepileptogenic effect of borneol in the pentylenetetrazole (PTZ)-induced kindling besides its ability to suppress oxidative stress and neuroinflammatory marker, glial fibrillary acidic protein (GFAP). Repeated administration of a subconvulsive dose of PTZ (35 mg/kg, i.p.) on every alternate day for 4 weeks produced kindling in mice. Borneol (5, 10, and 25 mg/kg, i.p.) and diazepam (1 mg/kg, i.p.) were given as a pretreatment prior to each PTZ injection during the progression of kindling. Oxidative stress parameters such as superoxide dismutase (SOD), reduced glutathione (GSH), catalase (CAT), and lipid peroxidation (LPO) were assessed at the end of the study. Neuronal damage was assessed by hematoxylin and eosin staining technique. GFAP was also evaluated in the hippocampus region of the brain by using immunohistochemistry. Borneol significantly suppressed the process of epileptogenesis in PTZ-kindled mice. The biochemical alterations induced by PTZ kindling were ameliorated in borneol-treated animals which was indicated by decreased LPO and increased SOD, GSH, CAT levels. The distinct neuronal damage observed in the kindled group was counteracted by borneol. Furthermore, it decreased the levels of GFAP which was manifested by reduced immunostaining. The above results are suggestive of the antiepileptogenic potential of borneol in the PTZ-induced kindling model of epilepsy, and thus, it could be a prospective molecule in the treatment of epilepsy.