6-shogaol attenuates H2O2-induced oxidative stress via upregulation of Nrf2-mediated γ-glutamylcysteine synthetase and heme oxygenase expression in HepG2 cells

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• 作者：Jin-Kyoung Kim ; Hae-Dong Jang
• 关键词：6 ; shogaol ; oxidative stress ; c ; jun N ; terminal kinase ; mediated ; γ ; glutamylcysteine synthetase ; heme oxygenase ; 1
• 刊名：Food Science and Biotechnology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：25
• 期：1
• 页码：319-327
• 全文大小：629 KB
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• 作者单位：Jin-Kyoung Kim (1)
  Hae-Dong Jang (1)
  
  1. Department of Food and Nutrition, Hannam University, Daejeon, 34054, Korea
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Nutrition
  Food Science
  
• 出版者：The Korean Society of Food Science and Technology in co-publication with Springer
• ISSN：2092-6456

文摘

The signaling pathway by which 6-shogaol protects HepG2 cells against H₂O₂-induced oxidative stress was investigated. Cellular anti-oxidant activities, the GSH level, and anti-oxidant response element (ARE) promoter activity were analyzed. Activated protein kinases and nuclear transcription factor-erythroid 2-related factor 2 (Nrf2) accumulation in the nucleus, and phase II detoxification and anti-oxidant enzymes were analyzed using western blotting. 6-Shogaol enhanced cellular anti-oxidant activities, the GSH level, and ARE promoter activities. Nrf2 accumulation in the nucleus, c-jun N-terminal kinase (JNK) activation, and γ-glutamylcysteine synthetase (GCS) and heme oxygenase-1 (HO-1) expressions were increased by 6-shogaol. Blockage of the JNK signaling pathway removed the elicitation effect of 6-shogaol on JNK activation, Nrf2 accumulation in nucleus, and GCS and HO-1 expression, but partially suppressed cellular anti-oxidant activities and ARE promoter activities. 6-shogaol exerts an indirect cellular anti-oxidant activity based on up-regulation of GCS and HO-1 via a JNK-mediated Nrf2 signaling pathway.