Preparation and characterization of heparin hexasaccharide library with N-unsubstituted glucosamine residues

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• 作者：Qun Tao Liang ; Jia Yan Du ; Qing Fu ; Jiang Hui Lin ; Zheng Wei
• 关键词：Heparin ; Hexasaccharide ; N ; unsubstituted disaccharide ; LC ; MS ; ITTOF
• 刊名：Glycoconjugate Journal
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：32
• 期：8
• 页码：643-653
• 全文大小：705 KB
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• 作者单位：Qun Tao Liang (1)
  Jia Yan Du (1)
  Qing Fu (1)
  Jiang Hui Lin (1)
  Zheng Wei (1)
  
  1. Institute of Glycobiochemistry, National Engineering Research Centre of Chemical Fertilizer Catalyst, Fu Zhou University, Fu Zhou, 350002, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-4986

文摘

The rare N-unsubstituted glucosamine (GlcNH₃ +) residues in heparan sulfate (HS) have important biological and pathophysiological roles. Therefore, the ability to chemically generate a series of oligosaccharides, which have a similar structure to the naturally-occurring, GlcNH₃ + - containing oligosaccharides from HS, would greatly contribute to investigating their natural role in HS. In this study, a hexasaccharide library that possess GlcNH₃ + residues were prepared from the chemical modification of the fully sulfated dp6. Chemical reaction conditions were optimized to generate different pattern of GlcNH₃ + - containing oligosaccharides, then the structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC/MS-ITTOF) analysis. EIC/MS and MS2 analysis showed different fragmentation patterns of dp6s with different GlcNH₃ + residues. This provides a foundation for further identification and quantification of GlcNH₃ + - oligosaccharides by mass spectrum analysis.