Effects of low-intensity ultrasound on cell proliferation and reproductivity

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• 作者：Chunmei Yang 杨春栿/a> ; Xuehui Jiang 蒋学惿/a> ; Kang Du 替帿/a>…
• 关键词：low ; intensity ultrasound ; cell viability ; cell morphology ; cell proliferation ; cell reproductivity
• 刊名：Transactions of Tianjin University
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：22
• 期：2
• 页码：125-131
• 全文大小：631 KB
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• 作者单位：Chunmei Yang 杨春梅 (1)
  Xuehui Jiang 蒋学慧 (1)
  Kang Du 杜 康 (1)
  Qiliang Cai 蔡启亮 (2)
  
  1. Tianjin Key Laboratory of Biomedical Detecting Techniques and Instruments, School of Precision Instrument and Opto-Electronics Engineering, Tianjin University, Tianjin, 300072, China
  2. Department of Urology, Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, Tianjin, 300211, China
  
• 刊物类别：Engineering
• 刊物主题：Chinese Library of Science
  
• 出版者：Tianjin University
• ISSN：1995-8196

文摘

Ultrasound has been widely used in clinics. Cellular responses to low-intensity ultrasound are parameter-dependent. Proper parameter setting is vital to its exact use. To get guidelines for parameter setting, lowintensity ultrasound stimulation on the proliferation and reproductivity of HepG2 and 3T3 cells in vitro was examined with a 1.06 MHz-generator by changing the parameters(including intensity, pulse repetition frequency and duty cycle)in a wide range. Cell viability and reproductivity at different time after sonication were measured by 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)and colony formation assay to indicate timerelated proliferation. The results illustrate that ultrasound irradiation at 0.4–0.8 W/cm2 and high pulse repetition frequency(100 Hz)can facilitate cell proliferation, while above 0.8 W/cm2 would resist it. The extent of resistance closely correlated with duty cycle and pulse repetition frequency. Resistance effect at low pulse repetition frequency(1 Hz)is greater than that at high pulse repetition frequency(100 Hz)and not time-related. The influence of high pulse repetition frequency is time-accumulated, indicating cellular process involved. These findings would provide valuable guidelines for the application of low-intensity ultrasound in stem cell transformation and tissue engineering.