Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica

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• 作者：Wenjing Zhang 张文雿/a> ; Delin Sun 孙德朿/a>…
• 关键词：sulfated polysaccharide ; HPLC post ; column derivatization ; microanalysis ; pharmacokinetics
• 刊名：Chinese Journal of Oceanology and Limnology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：34
• 期：1
• 页码：177-185
• 全文大小：523 KB
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• 作者单位：Wenjing Zhang 张文静 (1) (2)
  Delin Sun 孙德林 (3)
  Xia Zhao 赵峡 (4)
  Weihua Jin 金维华 (1)
  Jing Wang 王晶 (1)
  Quanbin Zhang 张全斌 (1)
  
  1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China
  2. University of Chinese Academy of Sciences, Beijing, 100049, China
  3. Heze Junxinyuan Food Company, Heze, 274415, China
  4. Ocean University of China, Qingdao, 266071, China
  
• 刊物主题：Oceanography;
• 出版者：Springer Berlin Heidelberg
• ISSN：1993-5005

文摘

A rapid, sensitive and reproducible high performance liquid chromatography (HPLC) method with post-column fluorescence derivatization has been developed to determine the amount of low-molecular-weight sulfated polysaccharide (GFS) in vivo. The metabolism of GFS has been shown to fit a two component model following its administration by intravenous injection, and its pharmacokinetic parameters were determined to be as follows: half-time of distribution phase (t _1/2α)=11.24±2.93 min, half-time of elimination phase (t _1/2β)=98.20±25.78 min, maximum concentration (C _max)=110.53 μg/mL and peak time (T _max)=5 min. The pharmacokinetic behavior of GFS was also investigated following intragastric administration. However, the concentration of GFS found in serum was too low for detection, and GFS could only be detected for up to 2 h after intragastric administration (200 mg/kg body weight). Thus, the bioavailability of GFS was low following intragastric administration because of the metabolism of GFS. In conclusion, HPLC with postcolumn derivatization could be used for quantitative microanalysis and pharmacokinetic studies to determine the presence of polysaccharides in the serum following intravenous injection.