Control of potato tuber dormancy and sprouting by expression of sense and antisense genes of pyrophosphatase in potato

详细信息    查看全文

• 作者：Huaijun Si ; Chongfen Zhang ; Ning Zhang ; Yikai Wen ; Di Wang
• 关键词：Pyrophosphatase ; Soluble sugars ; Tuber ; Dormancy ; Sprouting ; Potato
• 刊名：Acta Physiologiae Plantarum
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：38
• 期：3
• 全文大小：918 KB
• 参考文献：du Jardin P, Rojas-Beltran J, Cebhardt C, Brasseur R (1995) Molecular cloning and characterization of a soluble inorganic pyrophosphatase in potato. Plant Physiol 109:853–860CrossRef PubMed PubMedCentral
  Farré EM, Bachmann A, Willmitzer L, Trethewey RN (2001) Acceleration of potato sprouting by the expression of a bacterial pyrophosphatase. Nat Biotechnol 19:268–272CrossRef PubMed
  Hajirezaei M, Sonnewald U (1999) Inhibition of potato tuber sprouting: low levels of cytosolic pyrophosphate lead to non-sprouting tubers harvested from transgenic potato plants. Potato Res 42:353–372CrossRef
  Hajirezaei MR, Bornke F, Peisker M, Takahata Y, Lerchl J, Kirakosyan A, Sonnewald U (2003) Decreased sucrose content triggers starch breakdown and respiration in stored potato tubers (Solanum tuberosum). J Exp Bot 54:477–488CrossRef PubMed
  Heinonen JK, Lahti RJ (1981) A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pyrophosphatase. Anal Biochem 113:313–317CrossRef PubMed
  Jelitto T, Sonnewald U, Willmitzer L, Hajirezeai M, Stitt M (1992) Inorganic pyrophosphate content and metabolites in potato and tobacco plants expressing E. coli pyrophosphatase in their cytosol. Planta 188:238–244CrossRef PubMed
  Kieber JJ, Signer ER (1991) Cloning and characterization of an inorganic pyrophosphatase gene from Arabidopsis thaliana. Plant Mo1 Biol 16:345–348CrossRef
  Kim Y, Kim EJ, Rea PA (1994) Isolation and characterization of cDNAs encoding the vacuolar H+-pyrophosphatase of Beta vulgaris. Plant Physiol 106:375–382CrossRef PubMed PubMedCentral
  Lahti R, Pitkäranta T, Valve E, Ilta I, Kukko-Kalske E, Heinonen J (1988) Cloning and characterization of the gene encoding inorganic pyrophosphatase of Eschevichia coli K-12. J Bacteriol 170:5901–5907PubMed PubMedCentral
  Lang GA, Early JD, Martin GC, Darnell RL (1987) Endo-, para, and ecodormancy: physiological terminology and classification for dormancy research. HortScience 22:371–377
  Lerchl J, König S, Zrenner R, Sonnewald U (1995) Molecular cloning, characterization and expression analysis of isoforms encoding tonoplast-bound proton-translocating inorganic pyrophosphatase in tobacco. Plant Mol Biol 29:833–840CrossRef PubMed
  Li YZ, Liu HY, Zhang N, Si HJ, Wang D (2008) Study on the optimization of potato microtuber induction and its genetic transformation system. Biotechnology 18:65–68
  Liu HY, Li YZ, Liu N, Si HJ, Wang D (2008) Cloning of potato inorganic pyrophosphatase cDNA and construction of antisense plant expression vectors. Mol Plant Breed 6:131–135
  Sakakibara Y, Kobayashi H, Kasamo K (1996) Isolation and characterization of cDNAs encoding vacuolar H+-pyrophosphatase isoforms from rice (Oryza sativa L.). Plant Mol Biol 31:1029–1038CrossRef PubMed
  Shanghai Society for Plant Physiology (1985) Experimental manual of plant physiology. Shanghai Science and Technology Press, Shanghai, pp 136–138
  Sonnewald U (1992) Expression of E. coli inorganic pyrophosphatase in transgenic plants alters photoassimilates partitioning in leaves of transgenic plants. Plant J 1:95–100CrossRef
  Sonnewald U (2001) Control of potato tuber sprouting. Trends Plant Sci 6:333–335CrossRef PubMed
  Sonnewald S, Sonnewald U (2014) Regulation of potato tuber sprouting. Planta 239:27–38CrossRef PubMed
  Viola R, Pelloux J, van der Ploeg A, Gillespie T, Marquis N, Roberts AG, Hancock RD (2007) Symplastic connection is required for bud outgrowth following dormancy in potato (Solanum tuberosum L.) tubers. Plant Cell Environ 30:973–983CrossRef PubMed
  Visser K, Heimovaara-Dijkstra S, Kijne JW, Wang M (1998) Molecular cloning and characterization of an inorganic pyrophosphatase from barley. Plant Mol Biol 37:131–140CrossRef PubMed
  Weiner H, Stitt M, Heldt HW (1987) Subcellular compartmentalization of pyrophosphate and alkaline pyrophosphatase in leaves. Biochem Biophys Acta 893:13–21
  Zhang YC, Tian F (2007) Experimental and research methods of potato. Agricultural Science and Technology Publisher of China, Beijing, pp 152–153
  Zou Q (2000) Experimental guide for plant physiology. China Agriculture Press, Beijing, pp 113–114
  
• 作者单位：Huaijun Si (1) (2)
  Chongfen Zhang (1) (3)
  Ning Zhang (2)
  Yikai Wen (2)
  Di Wang (1)
  
  1. Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, 730070, People’s Republic of China
  2. College of Life Science and Technology, Gansu Agricultural University, Lanzhou, 730070, People’s Republic of China
  3. Suixian Seniar High School of Shangqiu City, Suixian, 476900, Henan, People’s Republic of China
  
• 刊物主题：Plant Physiology; Plant Genetics & Genomics; Plant Biochemistry; Plant Pathology; Plant Anatomy/Development; Agriculture;
• 出版者：Springer Berlin Heidelberg
• ISSN：1861-1664

文摘

Inorganic pyrophosphate (PPi) is an enzyme involved in sugar metabolism in potato tubers. In our previous study, we isolated an inorganic pyrophosphatase (PPase) gene from potato and obtained the transgenic potato plants transformed with the sense and antisense PPase genes respectively. In the present experiment, the physiological indexes, tuber dormancy, and sprouting characteristics of the transgenic potatoes were analyzed and evaluated. The result showed that the PPase activity and the inorganic phosphate content of tubers were lower in the antisense transgenic plant lines but were higher in the sense transgenic plant lines, compared with wild-type tubers. Soluble sugars, such as glucose, fructose and sucrose increased in transgenic plants that had overexpression of the sense PPase gene, but decreased in the antisense transgenic plant lines, compared with wild-type tubers. Tuber sprouting time of the antisense transgenic plants were delayed for 2 and 3 weeks and reached the 100 % sprouting rate only after 14 and 16 weeks storage compared with the wild-type when tubers are stored under 25 and 4 °C, respectively. In contrast, tuber sprouting time of the sense transgenic plants was earlier by approximately 2 weeks than that of wild-type tubers under these storage temperatures.