Subcellular dissemination of prothymosin alpha at normal physiology: immunohistochemical vis-a-vis western blotting perspective

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• 作者：Caroline Mwendwa Kijogi ; Christopher Khayeka-Wandabwa ; Keita Sasaki…
• 关键词：Prothymosin α ; Anti ; C terminal antibody ; Anti ; N terminal antibody ; Immunohistochemistry ; Western blotting and localization
• 刊名：BMC Physiology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：16
• 期：1
• 全文大小：3,466 KB
• 刊物主题：Human Physiology; Animal Physiology; Physiological, Cellular and Medical Topics; Entomology; Anatomy;
• 出版者：BioMed Central
• ISSN：1472-6793

文摘

Background The cell type, cell status and specific localization of Prothymosin α (PTMA) within cells seemingly determine its function. PTMA undergoes 2 types of protease proteolytic modifications that are useful in elucidating its interactions with other molecules; a factor that typifies its roles. Preferably a nuclear protein, PTMA has been shown to function in the cytoplasm and extracellularly with much evidence leaning on pathognomonic status. As such, determination of its cellular distribution under normal physiological context while utilizing varied techniques is key to illuminating prospective validation of its distinct functions in different tissues. Differential distribution insights at normal physiology would also portent better basis for further clarification of its interactions and proteolytic modifications under pathological conditions like numerous cancer, ischemic stroke and immunomodulation. We therefore raised an antibody against the C terminal of PTMA to use in tandem with available antibody against the N terminal in a murine model to explicate the differences in its distribution in brain cell types and major peripheral organs through western blotting and immunohistochemical approaches.