Combined effects of temperature and copper ion concentration on the superoxide dismutase activity in Crassostrea ariakensis

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• 作者：Hui Wang ; Hongshuai Yang ; Jiahui Liu ; Yanhong Li ; Zhigang Liu
• 关键词：Crassostrea ariakensis ; superoxide dismutase ; temperature ; copper ion concentration ; combined effect
• 刊名：Acta Oceanologica Sinica
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：35
• 期：4
• 页码：51-57
• 全文大小：231 KB
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• 作者单位：Hui Wang (1)
  Hongshuai Yang (2)
  Jiahui Liu (2)
  Yanhong Li (2)
  Zhigang Liu (2)
  
  1. School of Life Sciences, Huaiyin Normal University, Huaian, 223300, China
  2. Fisheries College, Guangdong Ocean University, Zhanjiang, 524088, China
  
• 刊物主题：Oceanography; Climatology; Ecology; Engineering Fluid Dynamics; Marine & Freshwater Sciences; Environmental Chemistry;
• 出版者：Springer Berlin Heidelberg
• ISSN：1869-1099

文摘

Superoxide dismutase (SOD) is a crucial antioxidant enzyme playing the first defense line in antioxidant pathways against reactive oxygen species in various organisms including marine invertebrates. There exist mainly two specific forms, Cu/Zn-SOD (SOD1) and Mn-SOD (SOD2), in eukaryotes. SODs are known to be concurrently modulated by a variety of environmental stressors. By using central composite experimental design and response surface method, the joint effects of water temperature (18–34°C) and copper ion concentration (0.1–1.5 mg/L) on the total SOD activity in the digestive gland of Crassostrea ariakensis were studied. The results showed that the linear effect of temperature was highly significant (P<0.01), the quadratic effect of temperature was significant (P<0.05); the linear effect of copper ion concentration was not significant (P>0.05), while the quadratic effect of copper ion concentration was highly significant (P<0.01); the interactive effect of temperature and copper ion concentration was not significant (P>0.05); the effect of temperature was greater than that of copper ion concentration. The model equation of digestive gland SOD enzyme activity towards the two factors of interest was established, with R2 and predictive R2 as high as 0.961 6 and 0.820 7, respectively, suggesting that the goodness-offit to experimental data be very satisfactory, and could be applied to prediction of digestive gland SOD activity in C. ariakensis under the conditions of the experiment. Our results would be conducive to addressing the health of aquatic animals and/or to detecting environmental problems by taking SOD as a potential bioindicator.