Molecular and cytogenetic characterization of a common wheat-Agropyron cristatum chromosome translocation conferring resistance to leaf rust

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• 作者：Virginia Ochoa (1) (2) (3)
  Eva Madrid (2)
  Mahmoud Said (1) (4)
  Diego Rubiales (2)
  Adoración Cabrera (1)
  
• 关键词：Common wheat ; Agropyron cristatum ; Introgression ; Leaf rust ; FISH ; GISH ; SSR markers
• 刊名：Euphytica
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：201
• 期：1
• 页码：89-95
• 全文大小：364 KB
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• 作者单位：Virginia Ochoa (1) (2) (3)
  Eva Madrid (2)
  Mahmoud Said (1) (4)
  Diego Rubiales (2)
  Adoración Cabrera (1)
  
  1. Department of Genetics, ETSIAM, University of Córdoba, Campus de Excelencia Internacional Agroalimentario, ceiA3, 14071, Córdoba, Spain
  2. Institute for Sustainable Agriculture, CSIC, Apdo 4084, 14080, Córdoba, Spain
  3. Canary Institute of Agri-Food Quality, Apdo 380, Santa Cruz de Tenerife, Spain
  4. Institute of Experimental Botany, Slechtitelu 31, 783 7, Olomouc-Holice, Czech Republic
  
• ISSN：1573-5060

文摘

Crested wheatgrass (Agropyron cristatum L. Gaertn.) is a perennial species of economic importance as forage that also displays potentially valuable traits for wheat improvement trough intergeneric hybridization. In order to incorporate resistance genes from A. cristatum against wheat leaf rust (Puccinia triticina Erikss.) into common wheat (Triticum aestivum L.) a breeding programme was carried out by crossing and backcrossing the self-fertile amphiploid AABBDDPP (2n?=?8x?=?56) with T. aestivum (2n?=?6x?=?42; AABBDD). The AABBDDPP amphiploid was previously obtained by crossing tetraploid wheat (Triticum turgidum L. conv. durum Desf. 2n?=?4x?=?28; AABB) with a self-fertile allotetraploid (2n?=?4x?=?28; DDPP) between diploid wheat (Aegilops tauschii Coss.) and crested wheatgrass (A. cristatum). After three backcrosses a fertile stable line (named TH4) was obtained with 42 chromosomes. The fluorescence in situ hybridization and GISH analysis confirmed that TH4 carries a compensating robertsonian translocation involving the long arm of wheat chromosome 1B and the short arm of an unidentified A. cristatum chromosome. Specific molecular markers from A. cristatum also demonstrated the presence of chromatin from this species in the TH4 line. Macroscopic and microscopic observations indicated that the A. cristatum fragment that has been transferred to common wheat contributed a substantial level of partial resistance to leaf rust. The A. cristatum translocation line in bread wheat makes the disease resistance gene(s) from A. cristatum accessible for wheat breeding programmes.