Identification of a Novel HOG1 Homologue from an Industrial Glycerol Producer Candida glycerinogenes

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• 作者：Hao Ji (1)
  Xinyao Lu (1)
  Chengyin Wang (1)
  Hong Zong (1)
  Huiying Fang (1)
  Jin Sun (2)
  Jian Zhuge (1)
  Bin Zhuge (1)
  
• 刊名：Current Microbiology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：69
• 期：6
• 页码：909-914
• 全文大小：425 KB
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• 作者单位：Hao Ji (1)
  Xinyao Lu (1)
  Chengyin Wang (1)
  Hong Zong (1)
  Huiying Fang (1)
  Jin Sun (2)
  Jian Zhuge (1)
  Bin Zhuge (1)
  
  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China
  2. Zhejiang Condiments Industry Research Center, Zhejiang Zhengwei Food Co., Ltd, Yiwu, China
  
• ISSN：1432-0991

文摘

Candida glycerinogenes, a glycerol production industrial strain with hyperosmo-adaptation can grow well in 15?% (w/v) NaCl or 55?% (w/v) glucose. To understand the osmo-adaptation mechanism in C. glycerinogenes, the mitogen-activated protein kinase HOG1 gene (CgHOG1), which plays an essential role in the yeast hyperosmotic response, was isolated by degenerate PCR and SEFA-Formed Adaptor PCR. The CgHOG1 gene was then transformed in Saccharomyces cerevisiae hog1Δ null mutant, which restored the recombination S. cerevisiae to the wild-type phenotype with osmo-adaptation. To further clarify the function of CgHOG1, the phosphorylation of CgHOG1 and transcription of the glycerol-3-phosphate dehydrogenase gene (GPD1) of the CgHOG1-harbouring S. cerevisiae mutant was detected, and found to be similar to that of wild-type S. cerevisiae. In addition, the recombination S. cerevisiae with CgHOG1 gene significantly accumulated intracellular glycerol when stressed with NaCl.