Huaier aqueous extract inhibits stem-like characteristics of MCF7 breast cancer cells via inactivation of hedgehog pathway

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• 作者：Xiaolong Wang (1)
  Ning Zhang (1)
  Qiang Huo (1)
  Mingjuan Sun (1)
  Lun Dong (1)
  Yan Zhang (2)
  Guangwei Xu (3)
  Qifeng Yang (1) (3)
  
• 关键词：Cancer stem cells ; Huaier extract ; Hedgehog ; Breast cancer ; MCF7
• 刊名：Tumor Biology
• 出版年：2014
• 出版时间：November 2014
• 年：2014
• 卷：35
• 期：11
• 页码：10805-10813
• 全文大小：2,745 KB
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• 作者单位：Xiaolong Wang (1)
  Ning Zhang (1)
  Qiang Huo (1)
  Mingjuan Sun (1)
  Lun Dong (1)
  Yan Zhang (2)
  Guangwei Xu (3)
  Qifeng Yang (1) (3)
  
  1. Department of Breast Surgery, Qilu Hospital, Shandong University, Wenhua Xi Road No. 107, Jinan, 250012, Shandong, People’s Republic of China
  2. Qilu Hospital, Shandong University, Wenhua Xi Road No. 107, Jinan, 250012, Shandong, People’s Republic of China
  3. Key Laboratory of Experimental Teratology, Ministry of Education and Institute of Molecular Medicine and Genetics, Shandong University School of Medicine, Wenhua Xi Road No. 44, Jinan, 250012, Shandong, People’s Republic of China
  
• ISSN：1423-0380

文摘

The theory of targeting cancer stem-like cells (CSCs) provides novel strategy for cancer treatment. In the present study, we examined the inhibitory effect of Huaier aqueous extract on eradicating breast cancer stem cells and explored the underlying mechanisms. Our data demonstrated that various concentrations of Huaier extract significantly decreased the viabilities, numbers, and sizes of mammospheres. After incubation with Huaier extract for 24?h, the clonogenicity of MCF7 cell line was obviously impaired, along with less holoclones. In addition, Huaier extract reduced the number of cells expressing CD44+/CD24?and decreased the level of stem cell markers (OCT-4, NESTIN, and NANOG). The hedgehog (Hh), notch, and Wnt/β-catenin pathways were essential stem cell signaling pathways involved in regulating CSC renewal and maintenance. We reported that the inhibitory effect of Huaier extract was partly depended on the inactivation of Hh pathway. These findings provided experimental evidence that Huaier extract was a promising therapeutic drug for eliminating the breast cancer stem cells.