The Relationship Between Serial [18?F]PBR06 PET Imaging of Microglial Activation and Motor Function Following Stroke in Mice

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• 作者：Frederick M. Lartey (1)
  G-One Ahn (6)
  Rehan Ali (1)
  Sahar Rosenblum (4)
  Zheng Miao (3)
  Natasha Arksey (3)
  Bin Shen (3)
  Marta Vilalta Colomer (1)
  Marjan Rafat (1)
  Hongguang Liu (3)
  Miguel A. Alejandre-Alcazar (5)
  John W. Chen (7)
  Theo Palmer (4)
  Frederick T. Chin (3)
  Raphael Guzman (4) (8)
  Billy W. Loo Jr. (1) (2)
  Edward Graves (1) (2)
  
• 关键词：Stroke ; [18?F]PBR06 PET imaging ; Microglia ; Neuroinflammation ; Mice ; Motor function ; Minocycline
• 刊名：Molecular Imaging and Biology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：16
• 期：6
• 页码：821-829
• 全文大小：3,991 KB
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  10. James ML, Fulton RR, Vercoullie J et al (2008) DPA-714, a new translocator protein-specific ligand: synthesis, radiofluorination, and pharmacologic characterization. J Nucl Med 49:814-22 CrossRef
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  14. James ML, Fulton RR, Henderson DJ et al (2005) Synthesis and / in vivo evaluation of a novel peripheral benzodiazepine receptor PET radioligand. Bioorg Med Chem 13:6188-194 CrossRef
  15. Yui J, Hatori A, Kawamura K et al (2011) Visualization of early infarction in rat brain after ischemia using a translocator protein (18?kDa) PET ligand [11C]DAC with ultra-high specific activity. Neuroimage 54:123-30 CrossRef
  16. Yui J, Maeda J, Kumata K et al (2010) 18?F-FEAC and 18?F-FEDAC: PET of the monkey brain and imaging of translocator protein (18?kDa) in the infarcted rat brain. J Nucl Med 51:1301-309 CrossRef
  17. Lartey FM
• 作者单位：Frederick M. Lartey (1)
  G-One Ahn (6)
  Rehan Ali (1)
  Sahar Rosenblum (4)
  Zheng Miao (3)
  Natasha Arksey (3)
  Bin Shen (3)
  Marta Vilalta Colomer (1)
  Marjan Rafat (1)
  Hongguang Liu (3)
  Miguel A. Alejandre-Alcazar (5)
  John W. Chen (7)
  Theo Palmer (4)
  Frederick T. Chin (3)
  Raphael Guzman (4) (8)
  Billy W. Loo Jr. (1) (2)
  Edward Graves (1) (2)
  
  1. Department of Radiation Oncology, Stanford University School of Medicine, Stanford, CA, USA
  6. Integrative Biosciences & Biotechnology, Pohang University of Science & Technology, Kyungbuk, South Korea
  4. Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA, USA
  3. Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA
  5. Department of Pediatrics, Stanford University School of Medicine, Stanford, CA, USA
  7. Center for Systems Biology and Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
  8. Departments of Neurosurgery and Biomedicine, University of Basel, Basel, Switzerland
  2. Stanford Cancer Institute, Stanford, CA, USA
  
• ISSN：1860-2002

文摘

Purpose Using [18?F]PBR06 positron emission tomography (PET) to characterize the time course of stroke-associated neuroinflammation (SAN) in mice, to evaluate whether brain microglia influences motor function after stroke, and to demonstrate the use of [18?F]PBR06 PET as a therapeutic assessment tool. Procedures Stroke was induced by transient middle cerebral artery occlusion (MCAO) in Balb/c mice (control, stroke, and stroke with poststroke minocycline treatment). [18?F]PBR06 PET/CT imaging, rotarod tests, and immunohistochemistry (IHC) were performed 3, 11, and 22?days poststroke induction (PSI). Results The stroke group exhibited significantly increased microglial activation, and impaired motor function. Peak microglial activation was 11?days PSI. There was a strong association between microglial activation, motor function, and microglial protein expression on IHC. Minocycline significantly reduced microglial activation and improved motor function by day 22 PSI. Conclusion [18?F]PBR06 PET imaging noninvasively characterizes the time course of SAN, and shows increased microglial activation is associated with decreased motor function.