Evaluation of protective immune response in mice by vaccination the recombinant adenovirus for expressing Schistosoma japonicum inhibitor apoptosis protein

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• 作者：Chao Hu (1)
  lihui Zhu (1)
  Rong Luo (1)
  Jinwei Dao (1)
  Jiangping Zhao (1)
  Yaojun Shi (1)
  Hao Li (1)
  Ke Lu (1)
  Xingang Feng (1)
  Jiaojiao Lin (1)
  Jinming Liu (1)
  Guofeng Cheng (1) (2)
  
• 关键词：Adenovirus ; Schistosoma japonicum ; Inhibitor apoptosis protein ; Vaccine
• 刊名：Parasitology Research
• 出版年：2014
• 出版时间：November 2014
• 年：2014
• 卷：113
• 期：11
• 页码：4261-4269
• 全文大小：754 KB
• 参考文献：1. Berriman M, Haas BJ, LoVerde PT, Wilson RA, Dillon GP, Cerqueira GC, Mashiyama ST, Al-Lazikani B, Andrade LF, Ashton PD, Aslett MA, Bartholomeu DC, Blandin G, Caffrey CR, Coghlan A, Coulson R, Day TA, Delcher A, DeMarco R, Djikeng A, Eyre T, Gamble JA, Ghedin E, Gu Y, Hertz-Fowler C, Hirai H, Hirai Y, Houston R, Ivens A, Johnston DA, Lacerda D, Macedo CD, McVeigh P, Ning Z, Oliveira G, Overington JP, Parkhill J, Pertea M, Pierce RJ, Protasio AV, Quail MA, Rajandream MA, Rogers J, Sajid M, Salzberg SL, Stanke M, Tivey AR, White O, Williams DL, Wortman J, Wu W, Zamanian M, Zerlotini A, Fraser-Liggett CM, Barrell BG, El-Sayed NM (2009) The genome of the blood fluke / Schistosoma mansoni. Nature 460(7253):352-58 CrossRef
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  14. Kamimura K, Suda T, Zhang
• 作者单位：Chao Hu (1)
  lihui Zhu (1)
  Rong Luo (1)
  Jinwei Dao (1)
  Jiangping Zhao (1)
  Yaojun Shi (1)
  Hao Li (1)
  Ke Lu (1)
  Xingang Feng (1)
  Jiaojiao Lin (1)
  Jinming Liu (1)
  Guofeng Cheng (1) (2)
  
  1. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, 518 Ziyue Road, Shanghai, 200241, China
  2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, 225009, China
  
• ISSN：1432-1955

文摘

Schistosomiasis is a worldwide parasitic disease, and while it can be successfully treated with chemotherapy, this does not prevent reinfection with the parasite. Adenovirus vectors have been widely used for vaccine delivery, and a vaccination approach has the potential to prevent infection with Schistosoma. Here, we developed a recombinant adenoviral vector that expresses Schistosoma japonicum inhibitor apoptosis protein (Ad-SjIAP) and assessed its immunoprotective functions against schistosomiasis in mice. Murine immune responses following vaccination were investigated using enzyme-linked immunosorbent assays (ELISA), lymphocyte proliferation, and cytokine assays. The protective immunity in mice was evaluated by challenging with S. japonicum cercariae. Our results indicated that immunization with the Ad-SjIAP in mice induced a strong serum IgG response against IAP including IgG1, IgG2a, and IgG2b. In addition, lymphocyte proliferation experiments showed that mice treated with Ad-SjIAP significantly increased the lymphocyte response upon stimulation with recombinant Schistosoma japonicum inhibitor apoptosis protein (rSjIAP). Moreover, cytokine assays indicated that vaccination of Ad-SjIAP significantly increased the production of interferon (IFN)-γ and IL-2 as compared to the corresponding control group. Furthermore, following the challenge with S. japonicum cercariae, the vaccine conferred moderate protection, with an average rate of 37.95?% for worm reduction and 31.7?% for egg reduction. Taken together, our preliminarily results suggested that schistosoma IAP may be a potential vaccine against S. japonicum and that adenoviral vectors may serve as an alternative delivery vehicle for schistosome vaccine development.