Identification of differentially expressed genes associated with differential body size in mandarin fish (Siniperca chuatsi)

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• 作者：Changxu Tian ; Ling Li ; Xu-Fang Liang ; Shan He ; Wenjie Guo ; Liyuan Lv…
• 刊名：Genetica
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：144
• 期：4
• 页码：445-455
• 全文大小：1,047 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Life Sciences
  Animal Genetics and Genomics
  Plant Genetics and Genomics
  Human Genetics
  Microbial Genetics and Genomics
  
• 出版者：Springer Netherlands
• ISSN：1573-6857
• 卷排序：144

文摘

Body size is an obvious and important characteristic of fish. Mandarin fish Siniperca chuatsi (Basilewsky) is one of the most valuable perciform species widely cultured in China. Individual differences in body size are common in mandarin fish and significantly influence the aquaculture production. However, little is currently known about its genetic control. In this study, digital gene expression profiling and transcriptome sequencing were performed in mandarin fish with differential body size at 30 and 180 days post-hatch (dph), respectively. Body weight, total length and body length of fish with big-size were significantly higher than those with small-size at both 30 and 180 dph (P < 0.05). 2171 and 2014 differentially expressed genes were identified between small-size and big-size fish at 30 and 180 dph, respectively. RT quantitative PCR (qPCR) analysis showed that the differential expression of 10 selected genes in mandarin fish that went through the same training procedure. The genes were involved in the growth hormone–insulin-like growth factor axis, cell proliferation and differentiation, appetite control, glucose metabolism, reproduction and sexual size dimorphism pathways. This study will help toward a comprehensive understanding of the complexity of regulation of body size in mandarin fish individuals and provide valuable information for future research.KeywordsBody sizeIndividual differenceMandarin fishDigital gene expression profilingTranscriptome sequencing