A novel transient phase kinetic analysis of the fractional modification of monocyclic enzyme cascades

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• 作者：Maria D. Masia ; Manuela Garcia-Moreno…
• 关键词：Enzyme kinetics ; Monocyclic cascades ; Transient phase ; Steady ; state ; Rapid equilibrium
• 刊名：Journal of Mathematical Chemistry
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：54
• 期：10
• 页码：1952-1972
• 全文大小：854 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Physical Chemistry
  Theoretical and Computational Chemistry
  Mathematical Applications in Chemistry
  
• 出版者：Springer Netherlands
• ISSN：1572-8897
• 卷排序：54

文摘

In this paper we analyse the transient phase, i.e., the time course, of the fractional modification of monocyclic enzyme cascades with a more complete realistic definition for fractional modification as the fraction of the original enzyme converted into the two forms of modified protein, and not only into the free modified protein. This same definition was recently used by our group in an analysis of only its steady state. Hence this contribution extends the above-mentioned one as it includes the transient phase, which provides much more information than a study into only the steady state. The fractional modification definition used herein is more complete than the definitions employed in previous works by other authors and ourselves, which only considered the fraction of molecules of the original enzyme converted into the free modified enzyme. By assuming a minimal set of linearising assumptions, we obtained a general transient phase equation for fractional modification. We also obtained the transient phase equation by assuming rapid equilibrium in the reversible steps involved in the scheme or the reaction. Both equations are valid for the four cyclic enzyme cascades studied herein. Their applicability and goodness were tested by the simulated time progress curves obtained by numerical integration. Finally, a kinetic data analysis is suggested.