Colorimetric determination of staphylococcal enterotoxin B via DNAzyme-guided growth of gold nanoparticles

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• 作者：Dandan Zhou ; Guoming Xie ; Xianqing Cao ; Xueping Chen ; Xing Zhang…
• 关键词：Plasmonic resonance ; Aptamer ; Hemin/G ; quadruplex ; Colorimetry ; Microplate assay ; Gold nanoparticle ; Staphylococcal enterotoxin B
• 刊名：Microchimica Acta
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：183
• 期：10
• 页码：2753-2760
• 全文大小：2,607 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Inorganic Chemistry
  Physical Chemistry
  Characterization and Evaluation Materials
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Wien
• ISSN：1436-5073
• 卷排序：183

文摘

The authors describe a colorimetric method for the determination of the staphylococcal enterotoxin B (SEB) that also allows for visual readout. The assay is based on the growth of gold nanoparticles (AuNPs) mediated by a hemin/G-quadruplex DNAzyme which generates a color change from red to blue in the presence of SEB. The method is enzyme-free and does not require a label. The kinetics of the formation of the AuNPs is controlled by the hemin/G-quadruplex DNAzyme and this is key to the signal generation mechanism. In the presence of SEB, the reactions between aptamer and target modulated the amount of single probe G strands that form DNAzyme capable of consuming hydrogen peroxide. The growth process of AuNPs is influenced by the resulting concentration of H₂O₂ and leads to the color change. Under optimal conditions, a linear relationship exists between absorbance and SEB concentration in the range from 0.1 to 500 pg·mL‾^{1 which covers the clinically relevant range. In case of visual detection, the lower limit of detection is 1 pg·mL−1. The assay described here is sensitive, comparably inexpensive and can detect SEB rapidly without the need for sophisticated equipment. In our perception, the method has a wide scope in that it may be adapted to various nucleic acids, proteins and other biomolecules if respective aptamers are available.}