Development of novel photopolymerizable hyaluronic acid/heparin-based hydrogel scaffolds with a controlled release of growth factors for enhanced bone regeneration

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• 作者：Min Soo Bae ; Na Re Ko ; Sang Jin Lee ; Jung Bok Lee…
• 关键词：photopolymerization ; hyaluronic acid ; heparin ; hydrogel ; growth factor ; bone tissue regeneration
• 刊名：Macromolecular Research
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：24
• 期：9
• 页码：829-837
• 全文大小：1,640 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Physical Chemistry
  Polymer Sciences
  Characterization and Evaluation of Materials
  Soft and Granular Matter, Complex Fluids and Microfluidics
  Nanochemistry
  Nanotec
  
• 出版者：The Polymer Society of Korea, co-published with Springer
• ISSN：2092-7673
• 卷排序：24

文摘

In this study, the effect of novel BMP-2-loaded photo-cured hydrogels on in vitro and in vivo osteogenesis were explored. The well-defined HA-heparin hydrogel was prepared by a photopolymerization of aminoethyl methacrylate (AEMA) grafted hyaluronic acid (HA) and AEMA-grafted heparin. Subsequently, the resulting hydrogels contained BMP-2 at different concentrations. First, a series of BMP-2-loaded hydrogels were characterized to determine their mechanical properties and release profiles. The results from rheology, swelling ratio, and release kinetics suggest that BMP-2 contained HA-g-Hhep gel (B/HA-g-Hhep gel) had sustained release during 4 weeks with minimal initial burst. Furthermore, the released BMP-2 maintained its biological activity. MTT and live/dead assays showed that the B/HA-g-Hhep gel had good biocompatibility to be used as a scaffold for bone regeneration. In vivo studies of B/HA-g-Hhep gel exhibited significantly enhanced osteogenesis, compared with other types of HA-heparin hydrogels. These excellent results indicated that B/HA-g-Hhep gel has a great potential for a controlled release of growth factor, leading to a good bone regeneration.Keywordsphotopolymerizationhyaluronic acidheparinhydrogelgrowth factorbone tissue regenerationReferences(1).J. W. Bae, D. H. Go, K. D. Park, and S. J. Lee, Macromol. Res., 14, 461 (2006).CrossRefGoogle Scholar(2).K. H. Hong, Y.-S. Jeon, D. J. Chung, and J.-H. Kim, Macromol. Res., 18, 204 (2010).CrossRefGoogle Scholar(3).J. Yun, A.-R. Oh, J. S. Im, H.-I. Kim, and Y.-S. Lee, Macromol. Res., 20, 1029 (2012).CrossRefGoogle Scholar(4).W.-Y. Lee, Y.-H. Chang, Y.-C. Yeh, C.-H. Chen, K. M. Lin, C.-C. Huang, Y. Chang, and H.-W. Sung, Biomaterials, 30, 5505 (2009).CrossRefGoogle Scholar(5).O. Jeon, S.-W. Kang, H.-W. Lim, D. Choi, D.-I. Kim, S. H. Lee, J. H. Chung, and B.-S. 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Durmaz, Polymer, 43, 1215 (2002).CrossRefGoogle ScholarCopyright information© The Polymer Society of Korea and Springer Sciene+Business Media Dordrecht 2016Authors and AffiliationsMin Soo Bae1Na Re Ko1Sang Jin Lee1Jung Bok Lee1Dong Nyoung Heo1Wook Byun1Byung-Joon Choi2Hoon Bong Jeon3Hee-Jae Jang4Ji-Yoon Ahn4Deok-Sang Hwang4Bock-Young Jung5Email authorIl Keun Kwon1Email author1.Department of Dental Materials & Institute of Oral Biology, School of DentistryKyung Hee UniversitySeoulKorea2.Department of Oral and Maxillofacial Surgery, School of DentistryKyung Hee UniversitySeoulKorea3.Department of Biomedical Engineering, College of MedicineKyung Hee UniversitySeoulKorea4.Department of Obstetrics & Gynecology, College of Korean MedicineKyung Hee UniversitySeoulKorea5.Department of Advanced General Dentistry, College of DentistryYonsei UniversitySeoulKorea About this article CrossMark Print ISSN 1598-5032 Online ISSN 2092-7673 Publisher Name The Polymer Society of Korea About this journal Reprints and Permissions Article actions function trackAddToCart() { var buyBoxPixel = new webtrekkV3({ trackDomain: "springergmbh01.webtrekk.net", trackId: "196033507532344", domain: "link.springer.com", contentId: "springer_com.buybox", product: "10.1007/s13233-016-4112-7_Development of novel photopolymeri", productStatus: "add", productCategory : { 1 : "ppv" }, customEcommerceParameter : { 9 : "link.springer.com" } }); buyBoxPixel.sendinfo(); } function trackSubscription() { var subscription = new webtrekkV3({ trackDomain: "springergmbh01.webtrekk.net", trackId: "196033507532344", domain: "link.springer.com", contentId: "springer_com.buybox" }); subscription.sendinfo({linkId: "inst. subscription info"}); } window.addEventListener("load", function(event) { var viewPage = new webtrekkV3({ trackDomain: "springergmbh01.webtrekk.net", trackId: "196033507532344", domain: "link.springer.com", contentId: "SL-article", product: "10.1007/s13233-016-4112-7_Development of novel photopolymeri", productStatus: "view", productCategory : { 1 : "ppv" }, customEcommerceParameter : { 9 : "link.springer.com" } }); viewPage.sendinfo(); }); Log in to check your access to this article Buy (PDF)EUR 34,95 Unlimited access to full article Instant download (PDF) Price includes local sales tax if applicable Find out about institutional subscriptions Export citation .RIS Papers Reference Manager RefWorks Zotero .ENW EndNote .BIB BibTeX JabRef Mendeley Share article Email Facebook Twitter LinkedIn Cookies We use cookies to improve your experience with our site. 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