Effects of tris(2,3-dibromopropyl) isocyanurate on steroidogenesis in H295R cells

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• 作者：Xun Li ; Yu Pan ; Chang Wang ; Minjie Chen ; Yuchen Liu ; Jia Li…
• 关键词：Tris(2 ; 3 ; dibromopropyl) isocyanurate ; H295R ; Sex hormone biosynthesis ; Steroidogenic gene ; Endocrine ; disrupting action
• 刊名：Environmental Earth Sciences
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：75
• 期：20
• 全文大小：844 KB
• 刊物类别：Earth and Environmental Science
• 刊物主题：None Assigned
• 出版者：Springer Berlin Heidelberg
• ISSN：1866-6299
• 卷排序：75

文摘

Tris(2,3-dibromopropyl) isocyanurate (TBC) is widely used in polymer products. It is ubiquitously found in environmental matrices. Previous studies have shown that TBC has potential endocrine-disrupting effects on aquatic organisms. However, the effects on adrenocortical function and the underlying mechanisms are not well characterized. In present study, the H295R cell line was employed to investigate the potential endocrine-disrupting action of TBC. TBC inhibited basal and forskolin-induced sex hormone production but did not exhibit cytotoxicity. The expression of the steroidogenic genes, StAR, 3βHSD2, CYP17, CYP21, CYP19, HMGR, 17βHSD1, 17βHSD4 and CYP11A, was not changed upon TBC exposure. However, the cAMP inducer forskolin strongly induced the expression of all these genes, except for HMGR, 17βHSD1 and 17βHSD4. TBC blocked forskolin-induced StAR, 3βHSD2, CYP17, CYP21 and CYP19 mRNA expression. Our results indicate that TBC can inhibit sex hormone biosynthesis due to the interference with steroidogenic gene transcription in activated condition.