Molecular diagnostics of the R81T mutation on the D-loop region of the β1 subunit of the nicotinic acetylcholine receptor gene conferring resistance to neonicotinoids in the cotton aphid, Aphis gossypii (Hemiptera: Aphididae)

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• 作者：Satoshi Toda ; Koichi Hirata ; Atsushi Yamamoto…
• 关键词：Multiplex polymerase chain reaction ; nAChR ; Point mutation ; Insecticide resistance ; Monitoring
• 刊名：Applied Entomology and Zoology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：52
• 期：1
• 页码：147-151
• 全文大小：
• 刊物主题：Entomology; Agriculture; Applied Ecology; Plant Pathology; Zoology; Environmental Management;
• 出版者：Springer Japan
• ISSN：1347-605X
• 卷排序：52

文摘

Aphis gossypii Glover is a cosmopolitan and polyphagous aphid species. When neonicotinoid-resistant clones of A. gossypii were recently found in Japan, it was subsequently discovered that a point mutation (R81T) in the D-loop region of the β1 subunit of the nicotinic acetylcholine receptor gene had conferred this resistance. To quickly identify this neonicotinoid-resistant allele, we developed a molecular diagnostics test using a multiplex polymerase chain reaction method that targeted the R81T mutation.