Identification of new -J and -K 16SrXII subgroups and distinct single nucleotide polymorphism genetic lineages among ‘Candidatus Phytoplasma solani’ strains associated with bois noir in Central Italy

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• 作者：F . Quaglino ; S. Murolo ; Y. Zhao ; P. Casati ; G. Durante…
• 关键词：Grapevine yellows ; 16S rDNA ; Virtual restriction fragment length polymorphism (RFLP) ; Sequence variants ; Ecological niches
• 刊名：Australasian Plant Pathology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：46
• 期：1
• 页码：31-34
• 全文大小：
• 刊物主题：Plant Pathology; Plant Sciences; Agriculture; Entomology; Ecology;
• 出版者：Springer Netherlands
• ISSN：1448-6032
• 卷排序：46

文摘

Bois noir of grapevines is attributed to infection by ‘Candidatus Phytoplasma solani’ strains, taxonomic subgroups 16SrXII-A, -F, and -G. Plant-to-plant transmission by polyphagous leafhoppers determined that ‘Ca. Phytoplasma solani’ strains exist in diverse plant hosts in varied ecosystems, where selection conceivably influences intra-species strain diversity. In this study, virtual restriction fragment length polymorphism (RFLP) and single nucleotide polymorphism (SNP) analyses of 16S rDNA nucleotide sequences, amplified from grapevines and bindweeds in central-eastern Italy, revealed the presence of two new subgroups (16SrXII-J and 16SrXII-K) in the 16SrXII group, and of genetically distinct SNP lineages. Findings from the present and related works report the extensive intra-species diversity among ‘Ca. Phytoplasma solani’ strains in vineyards, underscoring the need for further studies to investigate relationships between diverse phytoplasma strains and dynamics of bois noir epidemiology.