Role of Hydrogen Sulfide on Autophagy in Liver Injuries Induced by Selenium Deficiency in Chickens

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• 作者：Wang Wenzhong ; Zhang Tong ; Lin Hongjin ; Chang Ying…
• 关键词：Selenium deficiency ; Hydrogen sulfide ; Autophagy ; Chicken ; Liver
• 刊名：Biological Trace Element Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：175
• 期：1
• 页码：194-203
• 全文大小：
• 刊物主题：Biochemistry, general; Biotechnology; Nutrition; Oncology;
• 出版者：Springer US
• ISSN：1559-0720
• 卷排序：175

文摘

Selenium (Se) is an indispensable trace mineral that was associated with liver injuries in animal models. Hydrogen sulfide (H₂S) is involved in many liver diseases, and autophagy can maintain liver homeostasis with a stress stimulation. However, little is known about the correlation between H₂S and autophagy in the liver injury chicken models induced by Se deficiency. In this study, we aimed to investigate the correlation between H₂S and autophagy in the liver injury chicken models. We randomly divided 120 1-day-old chickens into two equal groups. The control group was fed with complete food with a Se content of 0.15 mg/kg, and the Se-deficiency group (lab group) was fed with a Se-deficient diet with a Se content of 0.033 mg/kg. When the time comes to 15, 25, and 35 days, the chickens were sacrificed (20 each). The liver tissues were gathered and examined for pathological observations, the mRNA and protein levels of H₂S synthases (CSE, CBS, and 3-MST) and the mRNA and protein levels of autophagy-related genes. The results showed that the expression of CSE, CBS, and 3-MST and H₂S production were higher in the lab group than in the control group. Swellings, fractures, and vacuolizations were visible in the mitochondria cristae in the livers of the lab group and autophagosomes were found as well. In addition, the expression of autophagy-related genes (ATG5, LC3-I, LC3-II, Beclin1, and Dynein) was higher in the lab group than in the control group (p < 0.05) while TOR decreased significantly in the lab group (p < 0.05). The results showed that H₂S and autophagy were involved in the liver injury chicken models, and H₂S was correlated with autophagy.