Long-term microfluidic tracking of coccoid cyanobacterial cells reveals robust control of division timing

详细信息    查看全文

• 作者：Feiqiao Brian Yu ; Lisa Willis ; Rosanna Man Wah Chau ; Alessandro Zambon…
• 关键词：Cyanobacteria ; Microfluidics ; Single ; cell imaging ; Light ; dark cycles ; Cell ; size homeostasis ; Circadian clock ; Photosynthesis
• 刊名：BMC Biology
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：15
• 期：1
• 全文大小：2467KB
• 刊物主题：Life Sciences, general;
• 出版者：BioMed Central
• ISSN：1741-7007
• 卷排序：15

文摘

BackgroundCyanobacteria are important agents in global carbon and nitrogen cycling and hold great promise for biotechnological applications. Model organisms such as Synechocystis sp. and Synechococcus sp. have advanced our understanding of photosynthetic capacity and circadian behavior, mostly using population-level measurements in which the behavior of individuals cannot be monitored. Synechocystis sp. cells are small and divide slowly, requiring long-term experiments to track single cells. Thus, the cumulative effects of drift over long periods can cause difficulties in monitoring and quantifying cell growth and division dynamics.