Screening of breast cancer stem cell inhibitors using a protein kinase inhibitor library

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• 作者：Hack Sun Choi ; Dal-Ah Kim ; Heesung Chung ; In Ho Park…
• 关键词：Cancer stem cell ; Mammosphere ; Protein kinase inhibitor ; Breast cancer
• 刊名：Cancer Cell International
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：17
• 期：1
• 全文大小：3287KB
• 刊物主题：Cancer Research; Cell Biology;
• 出版者：BioMed Central
• ISSN：1475-2867
• 卷排序：17

文摘

BackgroundCancer stem cells (CSCs), a subpopulation in tumors, are known to cause drug resistance, tumor recurrence and metastasis. Based on the characteristic formation of mammospheres in in vitro conditions, the mammosphere formation assay has become an essential tool for quantifying CSC activity in breast cancer research. However, manual counting of mammospheres is a time-consuming process that is not amenable to high-throughput screening, and there are occasional inaccuracies in the process of determining the mammosphere diameter. In this study, we proposed a novel automated counting method of mammosphere using the National Institute of Standards and Technology (NIST)’s Integrated Colony Enumerator (NICE) with a screening of protein kinase library.