Vitellogenin’s putative role in Tegillarca granosa’s cadmium detoxification

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• 作者：Caifang Chen ; Weiliang Shen ; Hailong Gu ; Linde Wu ; Zhihua Lin…
• 关键词：Cadmium ; Metallothionein ; Tegillarca granosa ; Temporal expression ; Vitellogenin
• 刊名：Genes & Genomics
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：39
• 期：2
• 页码：143-154
• 全文大小：
• 刊物主题：Microbial Genetics and Genomics; Plant Genetics & Genomics; Animal Genetics and Genomics; Human Genetics;
• 出版者：The Genetics Society of Korea
• ISSN：2092-9293
• 卷排序：39

文摘

The bloody clam, Tegillarca granosa, is a commercial benthic bivalve, having a strong accumulation ability and torrelence to cadmium. To investigate whether vitellogenin (Vg) is involved in cadmium (Cd) detoxification, the full-length cDNA of T. granosa Vg was cloned, and its expression pattern in response to cadmium exposure was studied compared with the reference metallothionein (MT) gene. The full T. granosa Vg sequence consisted of 8988 bp, including a 6930-bp open reading frame that encoded a 2309 amino acid polypeptide. The deduced Vg protein contained a Vg N-terminal domain, domain of unknown function (DUF1943), SbcC domain, and von Willebrand factor type D domain (VWD). Multiple metal-binding sites were predicted in the deduced T. granosa Vg protein, suggesting its potential in functioning as a metal-binding protein. In addition, Vg expression increased in the T. granosa digestive gland and hemolymph in time-dependent manner after exposure to 1, 3, 6 and 9 μg/L Cd for 28 days. MT expression was measured in parallel with Vg expression, and the latter was more sensitive to Cd induction than the former. Together, results of the present research suggested that Vg may play an important role in T. granosa metal detoxification.