Shoot multiplication of Syringa reticulata var. mandshurica from in vitro cultured seedlings

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• 作者：Chun-ping Liu ; Hua-ying Liu ; Bo-wen Yang ; Ling Yang…
• 关键词：Manchurian lilac ; Micropropagation ; Plant growth regulator ; Shoot multiplication ; Sucrose concentration
• 刊名：Journal of Forestry Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：28
• 期：1
• 页码：41-46
• 全文大小：
• 刊物主题：Forestry;
• 出版者：Springer Berlin Heidelberg
• ISSN：1993-0607
• 卷排序：28

文摘

We developed a shoot multiplication protocol for Syringa reticulata Blume var. mandshurica Hara from in vitro cultured seedlings that derived from in vitro germinated seeds. The shoots could be induced on Murashige and Skoog (MS) medium with proper plant growth regulator combinations of 6-benzylaminopurine (BA) and indole-3-butyric acid (IBA). The better medium for shoot multiplication and growth was MS + 5 mg L−1 BA + 0.5 mg L−1 IBA + 20 g L−1 sucrose + 7 g L−1 agar, and the corresponding shoot induction rate was 75 %. The plantlets grew well after rooting on 1/2MS medium (macro-elements of MS medium are at half-strength) supplemented with 1 mg L−1 IBA, and the survival percentage was >80 % at 16 weeks after transplanting.