A male-specific SCAR DNA marker and sex ratio of seedlings in salak (Salacca zalacca var. zalacca)

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• 作者：Rongsheng Li ; Jianguang Li ; Guangtian Yin ; Jinchang Yang…
• 关键词：Salacca ; Salak ; SCAR ; Sex
• 刊名：Journal of Forestry Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：28
• 期：1
• 页码：47-50
• 全文大小：
• 刊物主题：Forestry;
• 出版者：Springer Berlin Heidelberg
• ISSN：1993-0607
• 卷排序：28

文摘

A male-specific SCAR DNA marker was developed using a RAPD DNA marker specific for male plants of Salacca zalacca var. zalacca (salak palm). The marker is 1579 bp long and has a GC content of 38.5 %. Its sequence contains 1 or 2 open reading frames, indicating the marker is probably a coding region. No highly similar sequences were found in a search of the GenBank database. Sexes were identified using the SCAR DNA marker for three kinds of seedlings grouped by the number of seeds per fruit (1, 2 or 3). The sex ratio of female to male did not differ significantly from 1:1 for the three kinds of seedlings, implying that the number of seeds per fruit is not a reliable index to identify the sex of a seedling.