Bisphenol A promotes cholesterol absorption in Caco-2 cells by up-regulation of NPC1L1 expression

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• 作者：Dan Feng ; Jun Zou ; Shanshan Zhang ; Xuechun Li ; Peiyang Li…
• 关键词：Bisphenol A ; Cholesterol absorption ; Caco ; 2 cells ; Niemann ; Pick C1 ; like 1 ; Sterol regulatory element binding protein ; 2 ; Hypercholesterolemia
• 刊名：Lipids in Health and Disease
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：16
• 期：1
• 全文大小：602KB
• 刊物主题：Lipidology; Medical Biochemistry; Clinical Nutrition;
• 出版者：BioMed Central
• ISSN：1476-511X
• 卷排序：16

文摘

BackgroundBisphenol A (BPA), an commonly exposed environmental chemicals in humans, has been shown to have a hypercholesterolemic effect with molecular mechanism not clear. Since intestinal cholesterol absorption plays a major role in maintaining total body cholesterol homeostasis, the present study is to investigate whether BPA affects cholesterol absorption in the intestinal Caco-2 cells. Methods: The Caco-2 cells were pretreated with BPA at different concentrations for 24 h and then incubated with radioactive micellar cholesterol for 2 h. The absorption of radioactive cholesterol was quantified by liquid scintillation. The expression of Niemann-Pick C1-like 1 (NPC1L1) and sterol regulatory element binding protein-2 (SREBP-2) was analyzed by Western blot and qPCR.