The toxicity and DNA-damage mechanism of α-Fe₂O₃ nanoparticles

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• 作者：Xiaoxing Wang ; Yan Gu ; Davidmark Johnson ; Chuncheng Chen…
• 关键词：Toxicity ; DNA damage ; α ; Fe2O3 ; Mechanism
• 刊名：Medicinal Chemistry Research
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：26
• 期：2
• 页码：384-389
• 全文大小：
• 刊物主题：Pharmacology/Toxicology; Biochemistry, general; Cell Biology;
• 出版者：Springer US
• ISSN：1554-8120
• 卷排序：26

文摘

This study examined the toxicity of α-Fe₂O₃ nanoparticles and the mechanism of DNA photocatalytic oxidation on the surface of α-Fe₂O₃ nanoparticles irradiated by visible light. During oxidation, the extent of DNA damage was measured using high-performance liquid chromatography and the intermediates were identified by liquid chromatography–mass spectrometry. The concentration of H₂O₂ was determined using the catalytic method with horseradish peroxidase and N,N-diethyl-p-phenylenediamine (POD-DPD), and the concentration of OH free radicals was measured using fluorescence spectrophotometry. The results indicate that the reaction of OH free radicals generated from H₂O₂ by light-activated α-Fe₂O₃ nanoparticles is the primary cause of DNA damage and that the detected intermediates during the photocatalytic degradation of DNA were mainly produced by the hydroxylation of the DNA fragments. This work demonstrates that the α-Fe₂O₃ photocatalytic system provides a good model for investigating the toxicology of nanoparticles that produce OH free radicals after light activation. The toxicity of α-Fe₂O₃ nanoparticles is of concern to human health because of the damage it can cause to DNA.