Phenolic content, antioxidant potential and DNA damage protection of pearl millet (Pennisetum glaucum) cultivars of North Indian region

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• 作者：Raj Kumar Salar ; Sukhvinder Singh Purewal
• 关键词：Antioxidant activity ; Bioactive compounds ; Condensed tannin contents ; Pearl millet cultivars ; DNA protection
• 刊名：Journal of Food Measurement and Characterization
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：11
• 期：1
• 页码：126-133
• 全文大小：
• 刊物类别：Food Science; Chemistry/Food Science, general; Engineering, general;
• 刊物主题：Food Science; Chemistry/Food Science, general; Engineering, general;
• 出版者：Springer US
• ISSN：2193-4134
• 卷排序：11

文摘

Total phenolic contents, condensed tannin content, antioxidant activity and DNA damage protection of twelve pearl millet cultivars from North Indian region were assayed. All pearl millet cultivars showed considerable amount of bioactive compounds with antioxidant potential. PUSA-415 showed the presence of maximum amount of extractable total phenolic content (7.32 mg GAE/g DWB), whereas HHB-223 cultivar depicted the highest amount of condensed tannin contents (138.45 mg CE/100 g DWB). Antioxidant activity was measured using different methods viz., DPPH, ABTS, FRAP, TAC and HFRSA. A clear correlation was observed between total phenolic content and antioxidant activity of different pearl millet cultivars. The extracts of all cultivars prevented the oxidative damage to plasmid DNA (pBR322) against DNA damaging Fenton’s reagent. However, cultivars PC-383, PUSA-415, PUSA-605, PC-612 and 841-B showed prominent DNA damage protection activity. The results of this study provide a scientific basis of the traditional use of pearl millet as a natural antioxidant mitigating DNA damage.