Associating chemical analysis to molecular markers for the valorization of Citrus aurantium leaves: a useful starting point for marker-assisted selection

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• 作者：Myriam Lamine ; Fatma Zohra Rahali ; Ghaith Hamdaoui ; Sawsen Selmi ; Ahmed Mliki…
• 关键词：Antioxidant activity ; C. aurantium leaves ; Marker ; metabolite association ; Secondary metabolites ; SSR
• 刊名：Euphytica
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：213
• 期：2
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Plant Sciences; Plant Genetics & Genomics; Plant Pathology; Plant Physiology; Biotechnology;
• 出版者：Springer Netherlands
• ISSN：1573-5060
• 卷排序：213

文摘

Variation in metabolite composition and content is often observed in citrus, however, it is poorly understood to what extent this variation has a genetic basis. C. aurantium genotypes originating from Tunisia were evaluated to detect genomic (SSR markers) and chemotypic polymorphisms and to discover possible associations between them. A total of fifteen highly polymorphic SSR markers were selected to screen the genetic variability of the most widespread sour orange genotypes. Targeted secondary metabolite profiling analysis generated twenty-one compounds differentially accumulated in the leaves of sour orange genotypes. PCA analysis revealed that genomic and chemotypic data generated similar pattern of clustering, highlighting the intra-specific variability in C. aurantium species. Both data were integrated, leading to the identification of associated SSR alleles with secondary metabolites. Based on results, a relatively high correlation (r = 0.381; p < 0.0001) between chemotypic patterns and genetic markers was identified. Associations between traits of interest for phenolic compounds and genetic markers were tested using statistical methods including three linear model approaches. These results consolidate the presence of a chemical fingerprint that may be suitable for assessing identity and quality of a particular genotype which will be very useful for citrus breeding programs.