Mutant RoPI-1 allele-based marker development for selection of the hose-in-hose flower phenotype in Rhododendron obtusum cultivars

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• 作者：Kyeong-Seong Cheon ; Akira Nakatsuka ; Yu Gobara ; Nobuo Kobayashi
• 关键词：DNA selection marker ; Flower breeding ; Japanese azalea ; Multiplex ; PCR
• 刊名：Euphytica
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：213
• 期：1
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Plant Sciences; Plant Genetics & Genomics; Plant Pathology; Plant Physiology; Biotechnology;
• 出版者：Springer Netherlands
• ISSN：1573-5060
• 卷排序：213

文摘

To develop a gene-based DNA selection marker for the hose-in-hose flower phenotype, we used a simple multiplex-PCR approach to detect the RoPI-1 mutant allele in several azalea cultivars and their progenies. Amplified fragments approximately 470 and 730 bp in size were obtained from hose-in-hose phenotypes, whereas single flower phenotypes only presented the approximately 730-bp product; this was amplified from six Edo-kirishima cultivars, 46 Kurume azalea cultivars, and several progenies. In contrast, the hose-in-hose cultivars of Rhododendron indicum and Rhododendron transiens only presented the approximately 730-bp product. Therefore, our data indicated that multiplex-PCR might be used to select the hose-in-hose flower phenotype at the seedling stage, contributing to reduce breeding cycles, as Rhododendron obtusum cultivars takes 3–4 years to flower.