SCAR markers that discriminate between Dasypyrum species and cytotypes

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• 作者：M. Hu ; B. R. Baum ; D. A. Johnson
• 关键词：Cluster analysis ; Dasypyrum ; Principal component ; RAPD ; SCARs
• 刊名：Genetic Resources and Crop Evolution
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：64
• 期：3
• 页码：505-514
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Plant Sciences; Plant Systematics/Taxonomy/Biogeography; Plant Genetics & Genomics; Plant Physiology; Agriculture;
• 出版者：Springer Netherlands
• ISSN：1573-5109
• 卷排序：64

文摘

Dasypyrum is a genus with two species, an annual diploid Dasypyrum villosum and a perennial diploid or tetraploid D. breviaristatum. It is difficult to distinguish between the two, especially for a non-taxonomist. In this paper we demonstrate how to distinguish between the two species and between the two ploidy levels of D. breviaristatum. We achieve this using RAPD analysis to identify potential candidates for marker development and after carrying out principal component analyses and partitioning of genetic variance, developed SCARs and an identification key.