Effects of co-overexpression of the genes of Rubisco and transketolase on photosynthesis in rice

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• 作者：Yuji Suzuki ; Eri Kondo ; Amane Makino
• 关键词：Rubisco ; Transketolase ; Overproduction ; CO2 assimilation ; Rice
• 刊名：Photosynthesis Research
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：131
• 期：3
• 页码：281-289
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Plant Sciences; Plant Physiology; Biochemistry, general; Plant Genetics & Genomics;
• 出版者：Springer Netherlands
• ISSN：1573-5079
• 卷排序：131

文摘

Metabolome analyses have indicated an accumulation of sedoheptulose 7-phosphate in transgenic rice plants with overproduction of Rubisco (Suzuki et al. in Plant Cell Environ 35:1369–1379, 2012. doi:10.1111/j.1365-3040.2012.02494.x). Since Rubisco overproduction did not quantitatively enhance photosynthesis even under CO₂-limited conditions, it is suspected that such an accumulation of sedoheptulose 7-phosphate hampers the improvement of photosynthetic capacity. In the present study, the gene of transketolase, which is involved in the metabolism of sedoheptulose 7-phosphate, was co-overexpressed with the Rubisco small subunit gene in rice. Rubisco and transketolase were successfully overproduced in comparison with those in wild-type plants by 35–53 and 39–84 %, respectively. These changes in the amounts of the proteins were associated with those of the mRNA levels. However, the rate of CO₂ assimilation under high irradiance and different [CO₂] did not differ between co-overexpressed plants and wild-type plants. Thus, co-overproduction of Rubisco and transketolase did not improve photosynthesis in rice. Transketolase was probably not a limiting factor of photosynthesis as overproduction of transketolase alone by 80–94 % did not affect photosynthesis.