In vitro propagation of the endangered tree fern Cibotium barometz through formation of green globular bodies

详细信息    查看全文

• 作者：Rongpei Yu ; Guangfei Zhang ; Han Li ; Hua Cao…
• 关键词：Cibotium barometz ; In vitro propagation ; Green globular bodies ; Thidiazuron
• 刊名：Plant Cell, Tissue and Organ Culture (PCTOC)
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：128
• 期：2
• 页码：369-379
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Plant Sciences; Plant Physiology; Plant Genetics & Genomics; Plant Pathology;
• 出版者：Springer Netherlands
• ISSN：1573-5044
• 卷排序：128

文摘

Cibotium barometz is an endangered tree fern, used both as ornamental plant and traditional Chinese medicinal plant. In this study, an effective in vitro propagation protocol was obtained through formation of green globular bodies (GGBs) from in vitro juvenile sporophytes. The effect of plant growth regulators (PGRs) on GGB induction and multiplication, as well as mineral salt concentration and active charcoal (AC) on plantlet regeneration from GGBs was evaluated. Thidiazuron (TDZ; 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea) had a significant effect on GGB induction and multiplication (P < 0.001), while a-naphthaleneacetic acid (NAA) did not (P > 0.05). GGB induction rate was above 80 % on 1/2 Murashige and Skoog (MS) media supplemented with TDZ (1.0 mg L− 1) and NAA (0.1, 0.3 or 0.5 mg L− 1). The same media were also optimal for GGB multiplication. GGBs cultured on 1/4 MS media supplemented with 0.1 or 0.2 % (w/v) AC showed a high rate of GGB development into plantlets above 90 %. 1/2 MS media supplemented with 0.1 or 0.2 % AC were the most effective for plantlet growth. Regenerated plantlets were successfully acclimatized (80 %) in greenhouse conditions. Morphological and histological analysis revealed that C. barometz GGBs was a yellow-green globular structure composed of the single GGB with meristems and hair-like structures, and new single GGBs were initiated from the epidermal cells of meristem zone.