Isolation of four rice seed-specific promoters and evaluation of endosperm activity

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• 作者：Rongfang Xu ; Dongdong Li ; Hao Li ; Juan Li…
• 关键词：Endosperm specific ; Promoter ; Rice ; Transgene ; Biotechnology
• 刊名：Plant Cell, Tissue and Organ Culture (PCTOC)
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：128
• 期：1
• 页码：125-132
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Plant Sciences; Plant Physiology; Plant Genetics & Genomics; Plant Pathology;
• 出版者：Springer Netherlands
• ISSN：1573-5044
• 卷排序：128

文摘

Cereal grains are major targets for genetically improving the nutritional value of food and for producing recombinant proteins. Strong and tissue-specific promoters are highly desired for effectively controlling expression in the seed or endosperm. In this study, we isolated four rice promoters from the 5′ upstream region of putative seed-specifically expressed genes: PROLAM26, RAL2, RAL4 and CAPIP. By generating transgenic rice plants carrying promoter-reporter constructs, we found these four promoters to be specifically expressed in seeds, with three having endosperm-specific or -preferential activity. The strength of each promoter in the endosperm was determined and compared to a constitutively expressed OsACTIN promoter and an endosperm-specifically expressed Glu4-B promoter in single-copy transgenic plants. The promoter of RAL2 exhibited relatively high activity, and the promoters of RAL4 and CAPIP exhibited activities comparable with those of OsACTIN and Glu4-B. In addition, monitoring activities in high-generation (T₃–T₄) homozygous progeny of single-copy plants revealed maintenance of expression for all four promoters, with no evidence of silencing. Taken together, our findings offer four stable rice seed-specific promoters of different strengths for endosperm expression.