Fine mapping a major QTL qFCC7 _L for chlorophyll content in rice (Oryza sativa L.) cv. PA64s

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• 作者：Weijun Ye ; Shikai Hu ; Liwen Wu ; Changwei Ge ; Yongtao Cui…
• 关键词：Chlorophyll content ; Photosynthetic rate ; QTL analysis ; Low nitrogen condition ; Rice
• 刊名：Plant Growth Regulation
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：81
• 期：1
• 页码：81-90
• 全文大小：760KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Plant Sciences; Plant Anatomy/Development; Plant Physiology; Agriculture;
• 出版者：Springer Netherlands
• ISSN：1573-5087
• 卷排序：81

文摘

Chlorophyll (Chl) content is an important agronomic trait directly affecting the photosynthetic rate. Using a high-density genetic map of 132 recombinant inbred lines (RILs) derived from the cross between 93-11 and PA64s, we detected the quantitative trait loci (QTLs) for Chl content of the top three leaves under two nitrogen (N) conditions at two developmental stages. A total of 32 main-effect QTLs located on chromosomes 1, 4, 5, 6, 7, 8, and 12 were identified, and these QTLs individually accounted for 6.0–20.8 % of the total phenotypic variation. A major QTL qFCC7_L affecting the Chl content under low N condition was identified, and its positive allele came from PA64s. This QTL might be associated with the ability to tolerate low-N stress in rice. The chromosomal segment substitution line (CSSL) with the corresponding segment from PA64s had a higher SPAD value and photosynthetic rate than 93-11 and showed a lower specific leaf area (SLA). We performed a fine-mapping using a BC₄F₂ population via marker-assisted backcross and finally mapped this QTL to a 124.5 kb interval on the long arm of chromosome 7. Candidate gene analysis showed that there were sequence variations and expression differences in the predicted candidate gene between the two parents. These results suggest that the QTL qFCC7_L may be useful for breeding the rice varieties with higher photosynthetic rate and grain yield.