A gold nanoparticle-based colorimetric strategy coupled to duplex-specific nuclease signal amplification for the determination of microRNA

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• 作者：Hai-yan Shi ; Lang Yang ; Xiao-yu Zhou ; Jie Bai ; Jie Gao ; Hong-xia Jia…
• 关键词：AuNP ; miRNA ; DSNSA ; Photometric assay ; Salt ; induced aggregation
• 刊名：Microchimica Acta
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：184
• 期：2
• 页码：525-531
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Nanochemistry; Nanotechnology; Characterization and Evaluation of Materials; Analytical Chemistry; Microengineering;
• 出版者：Springer Vienna
• ISSN：1436-5073
• 卷排序：184

文摘

The authors report on a new gold nanoparticle-based colorimetric assay for microRNA (miRNA). It is based on duplex-specific nuclease (DSN)-assisted signal amplification. Following hybridization between target miRNA and its complementary single-stranded DNA (ssDNA), the ssDNA in the DNA:RNA hybrid is selectively cleaved by DSN to produce small DNA fragments. Target microRNA is thus released and will initiate another round of hybridization and DSN digestion. In this manner, each miRNA target can specifically trigger several cycles of hybridization and DSN cleavages to yield numerous small fragments of DNA oligonucleotides. The short DNA fragments (unlike the uncleaved ssDNA probe)  exhibit superior capability for stabilizing unmodified AuNP against salt-induced aggregation to the uncleaved ssDNA probe, with an accompanying color change from blue to red. These findings are exploited in a colorimetric assay for miRNA that has a 10 fmol detection limit.