Paper matrix based array for rapid and sensitive optical detection of mercury ions using silver enhancement

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• 作者：Li Yao ; Jun Teng ; Hao Qu ; Mengya Zhu ; Lei Zheng ; Feng Xue ; Wei Chen
• 关键词：Paper ; based analytical device ; Mercury ion detection ; Gold nanoparticle ; Colorimetry ; Silver deposition ; Signal amplification ; Nitrocellulose ; Image analysis
• 刊名：Microchimica Acta
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：184
• 期：2
• 页码：569-576
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Nanochemistry; Nanotechnology; Characterization and Evaluation of Materials; Analytical Chemistry; Microengineering;
• 出版者：Springer Vienna
• ISSN：1436-5073
• 卷排序：184

文摘

The authors describe a nitrocellulose membrane based paper matrix array for detection of mercury(II) with high throughput and repeatability. A thymine-rich signal reporting ssDNA probe labeled with gold nanoparticles (AuNPs) was designed, and detection is based on the strong T-Hg-T interaction which leads to a significant color change. In addition, catalytic signal amplification is accomplished by silver staining in order to enhance coloration. The method has a detection limit as low 0.5 ppt. This is at least 500-fold better than that of existing methods. By using an array, multiple sets of duplicate detections can be carried out simultaneously and in parallel. This improves the efficiency and accuracy of the assay. The method shows remarkable specificity since the signal of other competing metal ions is negligible compared to that of mercury(II) even at 100-fold higher concentration. The protocol was successfully applied to the determination of Hg(II) in spiked tap water and lake water samples. In our perception, this assay is a most promising tool for optical determination f ultra-low levels of Hg(II).