Heterologous expression of Aspergillus aculeatus endo-polygalacturonase in Pichia pastoris by high cell density fermentation and its application in textile scouring

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• 作者：Dede Abdulrachman ; Paweena Thongkred ; Kanokarn Kocharin…
• 关键词：Acidic pectinase ; Bioscouring ; Cotton fabric ; Heterologous expression ; High cell density fermentation
• 刊名：BMC Biotechnology
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：17
• 期：1
• 全文大小：680KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Applied Microbiology; Biotechnology; Biochemical Engineering; Genetic Engineering; Plant Breeding/Biotechnology; Biomedical Engineering/Biotechnology;
• 出版者：BioMed Central
• ISSN：1472-6750
• 卷排序：17

文摘

BackgroundRemoval of non-cellulosic impurities from cotton fabric, known as scouring, by conventional alkaline treatment causes environmental problems and reduces physical strength of fabrics. In this study, an endo-polygalacturonase (EndoPG) from Aspergillus aculeatus produced in Pichia pastoris was evaluated for its efficiency as a bioscouring agent while most current bioscouring process has been performed using crude pectinase preparation.