Lipid Rafts Promote trans Fatty Acid-Induced Inflammation in Human Umbilical Vein Endothelial Cells

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• 作者：Yao Pan ; Benxin Liu ; Zeyuan Deng ; Yawei Fan ; Jing Li ; Hongyan Li
• 关键词：trans Fatty acids ; Lipid rafts ; Inflammation ; NF ; kB ; ERK1/2
• 刊名：Lipids
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：52
• 期：1
• 页码：27-35
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Lipidology; Neurochemistry; Medical Biochemistry; Nutrition; Medicinal Chemistry; Microbial Genetics and Genomics;
• 出版者：Springer Berlin Heidelberg
• ISSN：1558-9307
• 卷排序：52

文摘

The effects of two fatty acids, oleic acid (OLA) and elaidic acid (ELA) on normal human umbilical vein endothelial cells (HUVEC) and non-rafts HUVEC were investigated in this study. The expression levels of inflammatory cytokines (ICAM-1, VCAM-1 and IL-6) were analyzed. Western blot was used to analyze the expression levels of inflammation-related proteins (NF-κB, ERK1/2) and toll-like receptors 4 (TLR4). The results showed that the levels of nuclear translocation of NF-κB p65 and phosphorylated ERK1/2 were significantly decreased only in non-lipid rafts cells pretreated with trans fatty acid (TFA). The expression of TLR4 in the ELA-treated normal cells was higher than that in non-lipid rafts HUVEC. When the lipid rafts was destroyed by methyl-β-cyclodextrin, the levels of nuclear translocation of NF-κB p65, phosphorylated ERK1/2 and TLR4 were decreased significantly. Therefore, lipid rafts may be involved in TFA induced-inflammation in HUVEC through blocking the inflammatory signal pathway. Lipid rafts might be a platform for specific receptors such as TLR4 for TFA to activate the pro-inflammation on cell membranes.