A sensitive aptasensor based on molybdenum carbide nanotubes and label-free aptamer for detection of bisphenol A

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• 作者：Meng-Qi He ; Kun Wang ; Jin Wang ; Yong-Liang Yu…
• 关键词：Biosensor ; Aptamer ; Label ; free ; Mo2C nanotubes ; Bisphenol A
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：409
• 期：7
• 页码：1797-1803
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Analytical Chemistry; Biochemistry, general; Laboratory Medicine; Characterization and Evaluation of Materials; Food Science; Monitoring/Environmental Analysis;
• 出版者：Springer Berlin Heidelberg
• ISSN：1618-2650
• 卷排序：409

文摘

To specifically and sensitively identify bisphenol A (BPA) with a simple and rapid method is very important for food safety. Using an anti-BPA aptamer and Mo₂C nanotubes, we developed a label-free and low-background signal biosensor for BPA detection. The anti-BPA aptamer drastically increased the fluorescence signal of N-methylmesoporphyrin IX under an assistance of Help-DNA. Additionally, BPA can interact with the anti-BPA aptamer and switch its conformation to prevent the formation of a G-quadruplex, resulting in fluorescence quenching. Simultaneously, Mo₂C nanotubes can reduce the background signals due to the adsorption of Help-DNA on their surface. This method shows a linear range of 2–20 nM with a detection limit of 2 nM for detecting BPA. This label-free BPA aptasensor with low background signal is inexpensive, easy to use, and can be applied to determine BPA in real water samples.