Enrichment of serum biomarkers by magnetic metal-organic framework composites

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• 作者：Ji-Ping Wei ; Heng Wang ; Tao Luo ; Zhi-Jiang Zhou…
• 关键词：MALDI ; TOF MS ; Enrichment ; Serum biomarker ; Magnetic metal ; organic framework composites
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：409
• 期：7
• 页码：1895-1904
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Analytical Chemistry; Biochemistry, general; Laboratory Medicine; Characterization and Evaluation of Materials; Food Science; Monitoring/Environmental Analysis;
• 出版者：Springer Berlin Heidelberg
• ISSN：1618-2650
• 卷排序：409

文摘

Highly efficient extraction of peptides from serum is critical for finding serum biomarkers using mass spectrometry, which still remains a great challenge. Currently, a bottom-up proteomics approach has been applied to discover serum biomarkers. However, the approach was labor intensive, time and cost consuming, and cannot meet the requirements for clinical application. In this work, Fe₃O₄/C@MIL-100 composites were synthesized to efficiently capture peptides from microwave-assisted formic acid digests of BSA and human serum prior to MALDI-TOF MS analysis. Fe₃O₄/C@MIL-100 composites exhibited size-selective adsorption performance, thus providing a rapid and convenient approach to enrich low-abundance peptides. Notably, the peptides’ mass fingerprinting of serum digestions between type 2 diabetes mellitus (T2DM) and healthy persons were distinguishable, which indicated the potential ability of this technique for T2DM diagnosis and rapid biomarker discovery.