MtTdp1α-depleted Medicago truncatula plants show reduced cuticle permeability and altered expression of defense genes

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• 作者：M. Donà ; M. E. Sabatini ; M. Biggiogera ; M. Confalonieri ; A. Minio…
• 关键词：Additional key wordscuticular waxes ; RNA ; Seq ; transgenic plants ; tyrosyl ; DNA phosphodiesterase
• 刊名：Biologia Plantarum
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：61
• 期：1
• 页码：192-196
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Plant Sciences;
• 出版者：Springer Netherlands
• ISSN：1573-8264
• 卷排序：61

文摘

The link between the MtTdp1α (tyrosyl-DNA phosphodiesterase) gene, involved in the repair of DNA topoisomerase I mediated DNA damage, and the plant defense response has been investigated in MtTdp1α-depleted Medicago truncatula transgenic lines obtained by intron-spliced hairpin RNA approach, compared to the control line (CTRL, empty vector). Reduction of cuticle permeability highlighted by chlorophyll efflux assays positively correlated with the level of MtTdp1α gene silencing. The increased cuticle thickness was confirmed by transmission electron microscopy, which revealed an apparent expansion of the epicuticular waxes deposited on the outer surface. RNA-Seq analysis, carried out in the MtTdp1α-depleted plants, revealed the different expression of resistance (R) genes, PAMP (pathogen-associated-molecular pattern) triggered immunity (PTI) genes and transcription factors (TFs) involved in the regulation of the plant defense response.