Aquamicrobium soli sp. nov., a bacterium isolated from a chlorobenzoate-contaminated soil

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• 作者：Chang-Feng Xu ; Long Zhang ; Jun-Wei Huang ; Kai Chen ; Shun-Peng Li…
• 关键词：Aquamicrobium soli sp. nov. ; Gram ; stain negative ; Taxonomy ; 16S rRNA gene
• 刊名：Antonie van Leeuwenhoek
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：110
• 期：3
• 页码：305-312
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Microbiology; Medical Microbiology; Plant Sciences; Soil Science & Conservation;
• 出版者：Springer International Publishing
• ISSN：1572-9699
• 卷排序：110

文摘

An aerobic, Gram-stain negative, short rod-shaped, asporogenous, non-motile bacterium designated strain NK8T was isolated from a chlorobenzoate contaminated soil in China. Strain NK8T was observed to grow optimally at pH 7.0, 30 °C and in the absence of NaCl in LB medium. The G + C content of the total DNA of strain NK8T was found to be 65.5 mol%. The 16S rRNA gene sequence of strain NK8T showed high similarity to that of Aquamicrobium aerolatum Sa14T (97.3%), followed by Aquamicrobium lusatiense S1T (96.7%) and Mesorhizobium sangali SCAU7T (96.6%). The DNA–DNA relatedness between strain NK8T and A. aerolatum Sa14T was 35.5 ± 0.9%. The major fatty acids of strain NK8T were determined to be C_19:0 cyclo ω8c (45.6%), C_18:1ω7c (33.4%) and C_16:0 (8.4%). The respiratory quinone was found to be ubiquinone Q-10. The major polyamine was found to be spermidine. The polar lipid profile include the major compounds phosphatidylcholine and diphosphatidylglycerol, and moderate amounts of phosphatidylethanolamine, phosphatidylmonomethylethanolamine, aminolipid and phospholipid. Based on the differential biochemical and physiological characteristics, the geno-, chemo- and phenotypic characteristics, strain NK8T is proposed to represent a novel species of the genus Aquamicrobium, Aquamicrobium soli sp. nov. The type strain is NK8T (=KCTC 52165T=CCTCC AB2016045T).