Histopathological and immunological changes during the acute and recovery phase in Henoch–Schönlein purpura rabbit model

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• 作者：Yanhong Li ; Xiaolong Sui ; Hua Zhu ; Yanfeng Xu…
• 关键词：Anaphylactoid purpura ; Acute and recovery phase ; Histopathology ; Immunology ; Rabbit model ; Vasculitis
• 刊名：Archives of Dermatological Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：309
• 期：1
• 页码：21-30
• 全文大小：
• 刊物类别：Medicine
• 刊物主题：Dermatology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1432-069X
• 卷排序：309

文摘

Henoch–Schönlein purpura (HSP) is a systemic vasculitis mediated by autologous immune complex. Animal models of HSP are scarce. Here, we describe the characteristics of HSP rabbit model in the acute and recovery phase. First, we constructed the HSP rabbit models, and then assessed immunologic indicators of models by enzyme-linked immunosorbent assay and immunoturbidimetry. Histomorphological characteristics were analyzed by haematoxylin–eosin, immunofluorescence and special staining. In the acute stage (24 h) after antigen challenge, the model group rabbits featured skin ecchymosis and abnormal laboratory examination results. Three weeks following the allergic reaction, purple spots improved markedly, and edema and blood seeping decreased, but obvious inflammation was present in the skin, kidneys, joints, gastrointestinal, lung and liver. Serological results of CD4, CD/CD8, IL-2, IL-4, and TNF-α, IgA, IgG, TropI, Alb and T were still abnormal. IgA and C3 expressed in skin and kidney and eosinophils expressed in skin and lungs were increased. The rabbit model can mimic human HSP lesions in symptoms, pathology, and immunology and may provide valuable insight into the pathogenesis of HSP and serve as a tool for future therapeutic development targeting HSP.