Rapid Method for Simultaneous Determination of Inositol Phosphates by IPC-ESI–MS/MS and Its Application in Nutrition and Genetic Research

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• 作者：Shaojun Zhang ; Wenzhu Yang ; Qianqian Zhao ; Xiaojin Zhou ; Yunliu Fan&#8230
• 关键词：Ion ; pair chromatography ; Liquid chromatography ; tandem mass spectrometry (LC–MS/MS) ; Inositol phosphates ; Dihexylammonium acetate (DHAA)
• 刊名：Chromatographia
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：80
• 期：2
• 页码：275-286
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chromatography; Proteomics; Pharmacy; Laboratory Medicine;
• 出版者：Springer Berlin Heidelberg
• ISSN：1612-1112
• 卷排序：80

文摘

Inositol phosphates (InsPs) have important biological functions and multiple nutritional effects. Breeding and nutrition studies of InsPs require a simple, rapid, and accurate method for high-throughput quantification. Here, we developed an ion-pair chromatography/tandem mass spectrometry (IPC/ESI–MS/MS) method for the simultaneous separation and determination of each InsP. A highly volatile ion-pair reagent (dihexylammonium acetate, DHAA) was applied to separate InsP₁–InsP₆, which were then quantified by multiple reaction monitoring (MRM) in negative ESI mode. This method could simultaneously detect InsP₁–InsP₆ within 15 min and exhibited a wide linearity (typically 0.3–1200 pmol). The lower limit of detection was 0.3 pmol for all InsPs, excluding InsP₂ (0.15 pmol) and InsP₆ (3 pmol). The method accuracy of all analytes ranged between 87 and 111% with the inter- and intra-day precision of 0.9–15 and 2.2–11%, respectively. This method was successfully applied to quantitate InsPs in different types of crop seeds, organs, and a maize inbred germplasm collection composed of hundreds of inbred lines, showing its potential for promoting the nutrition and genetic research of InsPs.