A simple and reliable analytical method based on HPLC–UV to determine oleanonic acid content in Chios gum mastic for quality control

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• 作者：Yan Jin ; Jing Zhao ; Kyung Min Jeong ; Da Eun Yoo…
• 关键词：Chios gum mastic ; Oleanonic acid ; Method validation ; Quality control
• 刊名：Archives of Pharmacal Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：40
• 期：1
• 页码：49-56
• 全文大小：
• 刊物主题：Pharmacy; Pharmacology/Toxicology;
• 出版者：Pharmaceutical Society of Korea
• ISSN：1976-3786
• 卷排序：40

文摘

A reliable analytical method based on high-performance liquid chromatography-ultraviolet detection was established for the determination of oleanonic acid (OA) content in Chios gum mastic (CGM). A simple method involving methanol extraction of CGM powder followed by basification and ether extraction was developed to isolate the triterpenic fraction including OA. The triterpenic fraction was chromatographed on a Phenomenex Gemini C₁₈ column (150 × 4.6 mm, 5 µm) under a simple gradient elution of a mobile phase containing acetonitrile and water at a flow rate of 1.0 mL min−1. The detection wavelength was set at 210 nm. Good linearity was achieved in the range of 100.0–1000.0 μg mL−1 with r2 > 0.9993, and the limit of quantification was 32.22 μg mL−1. Accuracy measured at three concentration levels was in the range of 93.72–99.56%, while intra-day and inter-day precisions estimated using both OA standard and CGM samples were no more than 2.83 and 4.57% RSD, respectively. Finally, this method was applied to real CGM samples from various batches, revealing that the OA contents were between 88.13 and 100.83 μg mg−1. These results suggest that the current method can be applied as an efficient analytical method for quality control of CGM.