Auxin Extraction and Purification Based on Recombinant Aux/IAA Proteins

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• 作者：Yi Su ; Weigui Luo ; Xiaofei Chen ; Huizhen Liu ; Yueqing Hu…
• 关键词：IAA ; Extraction and Purification ; Binding Capability ; Aux/IAA ; SPE
• 刊名：Biological Procedures Online
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：19
• 期：1
• 全文大小：3595KB
• 刊物主题：Biomedicine general; Biological Techniques;
• 出版者：BioMed Central
• ISSN：1480-9222
• 卷排序：19

文摘

BackgroundIndole-3-acetic acid (IAA) extraction and purification are of great importance in auxin research, which is a hot topic in the plant growth and development field. Solid-phase extraction (SPE) is frequently used for IAA extraction and purification. However, no IAA-specific SPE columns are commercially available at the moment. Therefore, the development of IAA-specific recognition materials and IAA extraction and purification methods will help researchers meet the need for more precise analytical methods for research on phytohormones.