Screening white spot syndrome virus (WSSV)-resistant molecular markers from Fenneropenaeus chinensis

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• 作者：Yingying Wu ; Xianhong Meng ; Jie Kong ; Sheng Luan…
• 关键词：Key wordsFenneropenaeu chinensis ; WSSV ; SNP ; molecular marker
• 刊名：Journal of Ocean University of China
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：16
• 期：1
• 页码：129-136
• 全文大小：
• 刊物主题：Oceanography; Meteorology;
• 出版者：Science Press
• ISSN：1993-5021
• 卷排序：16

文摘

White spot syndrome virus (WSSV)-resistant molecular markers were screened from the selectively bred new variety ‘Huanghai No. 2’ of Fenneropenaeus chinensis using unlabeled-probe high-resolution melting (HRM) technique. After the artificial infection with WSSV, the first 96 dead shrimps and the last 96 surviving shrimps were collected, representing WSSV-susceptible and -resistant populations, respectively. The genotypes at well-developed 39 single nucleotide polymorphisms (SNPs) loci were obtained. As revealed in the Chi-square test, 3 SNPs, genotype A/A of contig C364-89AT, genotype A/A of C2635-527CA and genotype C/T of contig C12355-592CT, were positively correlated with disease-resistance traits. Other 2 SNPs, genotype G/G of contig C283-145AG and genotype C/C of contig C12355-592CT, were negatively correlated. Moreover, analysis with BlastX program for disease-resistant SNPs indicated that 3 contigs, Contig283, Contig364 and Contig12355, matched to the functional genes of effector caspase of Penaeus monodon, peptide transporter family 1-like protein, and 40S ribosomal protein S2 of Perca flavescens with high sequence similarity. The results will be helpful to provide theoretical and technical supports for molecular marker-assisted selective breeding of F. chinensis.